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Title: Fabrication of mineralized polymeric nanofibrous composites for bone graft materials
Authors: Ngiam, M.
Liao, S. 
Patil, A.J.
Cheng, Z. 
Yang, F.
Gubler, M.J.
Ramakrishna, S. 
Chan, C.K. 
Issue Date: 1-Mar-2009
Citation: Ngiam, M., Liao, S., Patil, A.J., Cheng, Z., Yang, F., Gubler, M.J., Ramakrishna, S., Chan, C.K. (2009-03-01). Fabrication of mineralized polymeric nanofibrous composites for bone graft materials. Tissue Engineering - Part A 15 (3) : 535-546. ScholarBank@NUS Repository.
Abstract: Poly-L-lactic acid (PLLA) and PLLA/collagen (50% PLLA+50% collagen; PLLA/Col) nanofibers were fabricated using electrospinning. Mineralization of these nanofibers was processed using a modified alternating soaking method. The structural properties and morphologies of mineralized PLLA and PLLA/Col nanofibers were investigated using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and contact angle measurements. Human bone-derived osteoblasts were cultured on the materials for up to 1 week to assess the biological properties of the nanofibrous composites. Cell attachment on these nanocomposites was also tested within 1 h of culture at room temperature. The mechanical properties of the cell-nanocomposite constructs were determined using tensile testing. From our results, the bone-like nano-hydroxyapatite (n-HA) was successfully deposited on the PLLA and PLLA/Col nanofibers. We observed that the formation of n-HA on PLLA/Col nanofibers was faster and significantly more uniform than on pure PLLA nanofibers. The n-HA significantly improved the hydrophilicity of PLLA/Col nanofibers. From the results of cell attachment studies, n-HA deposition enhanced the cell capture efficacy at the 20-minute time point for PLLA nanofibers. The E-modulus values for PLLA+n-HA with cells (day 1 and day 4) were significantly higher than for PLLA+n-HA without cells. Based on these observations, we have demonstrated that n-HA deposition on nanofibers is a promising strategy for early cell capture. © Copyright 2009, Mary Ann Liebert, Inc. 2009.
Source Title: Tissue Engineering - Part A
ISSN: 19373341
DOI: 10.1089/ten.tea.2008.0011
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