Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.biomaterials.2009.02.025
DC Field | Value | |
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dc.title | Differentiation of bone marrow-derived mesenchymal stem cells into multi-layered epidermis-like cells in 3D organotypic coculture | |
dc.contributor.author | Ma, K. | |
dc.contributor.author | Laco, F. | |
dc.contributor.author | Ramakrishna, S. | |
dc.contributor.author | Liao, S. | |
dc.contributor.author | Chan, C.K. | |
dc.date.accessioned | 2014-06-17T06:17:30Z | |
dc.date.available | 2014-06-17T06:17:30Z | |
dc.date.issued | 2009-07 | |
dc.identifier.citation | Ma, K., Laco, F., Ramakrishna, S., Liao, S., Chan, C.K. (2009-07). Differentiation of bone marrow-derived mesenchymal stem cells into multi-layered epidermis-like cells in 3D organotypic coculture. Biomaterials 30 (19) : 3251-3258. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2009.02.025 | |
dc.identifier.issn | 01429612 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/59954 | |
dc.description.abstract | The interactions of bone marrow-derived mesenchymal stem cells (MSCs) and their engrafted microenvironment are an integral part of signaling control of stem cell lineage commitment. We attempted to induce bone marrow-derived MSCs to undergo epidermal lineage differentiation by manipulating the biochemical, environmental and physical properties of culture conditions in an organotypic coculture model to simulate a skin-specific microenvironment. The induction medium was optimized by varying different biomolecular supplements in a basic stratification medium. A multi-layered epidermis-like structure was established when MSCs were cultured in an optimized induction medium on a contractible fibroblast-embedded collagen gel with an air-liquid interface. The commitment into epidermal lineage was further confirmed by the expression of early and intermediate epidermalization markers - keratin 10 and filaggrin in 90.67% and 80.51% of MSCs, respectively. This study not only highlights the possibility of in vitro control of MSCs into epidermal lineage, but also suggests the therapeutic potential of bone marrow-derived MSCs for skin regeneration. © 2009 Elsevier Ltd. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2009.02.025 | |
dc.source | Scopus | |
dc.subject | Epidermal differentiation | |
dc.subject | Mesenchymal stem cells | |
dc.subject | Organotypic coculture | |
dc.subject | Skin regeneration | |
dc.type | Article | |
dc.contributor.department | BIOENGINEERING | |
dc.contributor.department | ORTHOPAEDIC SURGERY | |
dc.contributor.department | MECHANICAL ENGINEERING | |
dc.description.doi | 10.1016/j.biomaterials.2009.02.025 | |
dc.description.sourcetitle | Biomaterials | |
dc.description.volume | 30 | |
dc.description.issue | 19 | |
dc.description.page | 3251-3258 | |
dc.description.coden | BIMAD | |
dc.identifier.isiut | 000266671000005 | |
Appears in Collections: | Staff Publications |
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