Please use this identifier to cite or link to this item: https://doi.org/10.1089/ten.2006.12.2457
DC FieldValue
dc.titleBiodegradable polymer nanofiber mesh to maintain functions of endothelial cells
dc.contributor.authorHe, W.
dc.contributor.authorYong, T.
dc.contributor.authorMa, Z.W.
dc.contributor.authorInai, R.
dc.contributor.authorTeo, W.E.
dc.contributor.authorRamakrishna, S.
dc.date.accessioned2014-06-17T06:13:44Z
dc.date.available2014-06-17T06:13:44Z
dc.date.issued2006-09
dc.identifier.citationHe, W., Yong, T., Ma, Z.W., Inai, R., Teo, W.E., Ramakrishna, S. (2006-09). Biodegradable polymer nanofiber mesh to maintain functions of endothelial cells. Tissue Engineering 12 (9) : 2457-2466. ScholarBank@NUS Repository. https://doi.org/10.1089/ten.2006.12.2457
dc.identifier.issn10763279
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/59631
dc.description.abstractMaintaining functions of endothelial cells in vitro is a prerequisite for effective endothelialization of biomaterials as an approach to prevent intimal hyperplasia of small-diameter vascular grafts. The aim of this study was to design suitable nanofiber meshes (NFMs) that further maintain the phenotype and functions of human coronary artery endothelial cells (HCAECs). Collagen-coated random and aligned poly(L-lactic acid)-co-poly(ε-caprolactone) (P(LLA-CL)) NFMs were fabricated using electrospinning. Mechanical testing showed that tensile modulus and strength were greater for the aligned P(LLA-CL) NFM than for the random NFM. Spatial distribution of the collagen in the NFMs was visualized by labeling with fluorescent dye. HCAECs grew along the direction of nanofiber alignment and showed elongated morphology that simulated endothelial cells in vivo under blood flow. Both random and aligned P(LLA-CL) NFMs preserved phenotype (expression of platelet endothelial cell adhesion molecule-1, fibronectin, and collagen type IV in protein level) and functions (complementary DNA microarray analysis of 112 genes relevant to endothelial cell functions) of HCAECs. The P(LLA-CL) NFMs are potential materials for tissue-engineered vascular grafts that may enable effective endothelialization. © Mary Ann Liebert, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1089/ten.2006.12.2457
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentNUS NANOSCIENCE & NANOTECH INITIATIVE
dc.contributor.departmentBIOENGINEERING
dc.contributor.departmentMECHANICAL ENGINEERING
dc.description.doi10.1089/ten.2006.12.2457
dc.description.sourcetitleTissue Engineering
dc.description.volume12
dc.description.issue9
dc.description.page2457-2466
dc.description.codenTIENF
dc.identifier.isiut000240780900006
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

114
checked on Nov 27, 2020

WEB OF SCIENCETM
Citations

105
checked on Nov 27, 2020

Page view(s)

133
checked on Dec 1, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.