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Title: Characterization of p53 gene mutations in colorectal carcinomas from an Asian population
Authors: Shi, C.-Y.
Phang, T.-W.
Ngoi, S.-S.
Wee, A.
Li, B. 
Lin, Y.
Lee, H.-P. 
Ong, C.-N. 
Keywords: Colorectal carcinoma
Ethnic differences
p53 gene
Tumor site
Issue Date: 1994
Citation: Shi, C.-Y.,Phang, T.-W.,Ngoi, S.-S.,Wee, A.,Li, B.,Lin, Y.,Lee, H.-P.,Ong, C.-N. (1994). Characterization of p53 gene mutations in colorectal carcinomas from an Asian population. Anticancer Research 14 (6 B) : 2811-2816. ScholarBank@NUS Repository.
Abstract: A series of colorectal carcinomas from an Asian population in Singapore were analyzed for mutations in the tumor suppressor gene p53. Based on single-stand conformation polymorphism (PCR-SSCP) analysis and direct DNA sequencing, 15 of 38 tumors (39%) were found to contain mutations in exons 5-8 of the p53 gene. The point mutations were predominantly base transitions. Among the 10 transitions, 5 were at CpG dinucleotide sites. One-third (5/15) of the mutations were found at previously identified hotspot codons 175, 248 and 282. In one case an insertion of a 6-base pair sequence was found. p53 mutations did not correlate with tumor histological grade, Dukes' stage, or metastases. However, tumors at the distal site showed a higher proportion of mutations than the proximal site. Further, no mutation was found in the normal mucosa adjacent to tumor site, suggesting that no germ-line mutations were present. The results were compared with those from other studies and are discussed in connection to possible etiological factors that are specific to the local population.
Source Title: Anticancer Research
ISSN: 02507005
Appears in Collections:Staff Publications

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