Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/53261
DC FieldValue
dc.titleYeast expression and characterization of SARS-CoV N protein
dc.contributor.authorCao, S.
dc.contributor.authorWang, H.
dc.contributor.authorLuhur, A.
dc.contributor.authorWong, S.-M.
dc.date.accessioned2014-05-19T02:56:20Z
dc.date.available2014-05-19T02:56:20Z
dc.date.issued2005-12
dc.identifier.citationCao, S., Wang, H., Luhur, A., Wong, S.-M. (2005-12). Yeast expression and characterization of SARS-CoV N protein. Journal of Virological Methods 130 (1-2) : 83-88. ScholarBank@NUS Repository.
dc.identifier.issn01660934
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/53261
dc.description.abstractThe severe acute respiratory syndrome human coronavirus (SARS-CoV) nucleocapsid protein (N protein) is its most antigenic structural protein. The N protein gene has been cloned into a yeast expression vector pPIC9, transformed into Pichia pastoris strain GS115 and induced for expression by methanol. SDS-PAGE and Western blot showed that the N protein was expressed at a level of 3 mg/ml of culture medium. Characterization by mass spectrometry, circular dichroism and fluorescence luminescence assays showed that the expressed N protein displayed a β-sheet secondary structure in solution and it is stable in the pH range between 5.0 and 8.0. The P. pastoris-expressed N protein is believed to more closely resemble native SARS N protein than the bacterially expressed N protein. © 2005 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jviromet.2005.06.010
dc.sourceScopus
dc.subjectN protein
dc.subjectPichia pastoris
dc.subjectSARS-CoV
dc.subjectYeast expression
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.sourcetitleJournal of Virological Methods
dc.description.volume130
dc.description.issue1-2
dc.description.page83-88
dc.description.codenJVMED
dc.identifier.isiut000233499100013
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

2
checked on Feb 27, 2018

WEB OF SCIENCETM
Citations

2
checked on Dec 31, 2018

Page view(s)

125
checked on Apr 13, 2021

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.