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Title: Serological response in RT-PCR confirmed h1n1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: An observational study
Authors: Chen, M.I. 
Barr, I.G.
Koh, G.C.H. 
Lee, V.J. 
Lee, C.P.S.
Shaw, R.
Lin, C.
Yap, J.
Cook, A.R. 
Tan, B.H.
Loh, J.P.
Barkham, T.
Chow, V.T.K.
Lin, R.T.P.
Leo, Y.-S.
Issue Date: 2010
Citation: Chen, M.I., Barr, I.G., Koh, G.C.H., Lee, V.J., Lee, C.P.S., Shaw, R., Lin, C., Yap, J., Cook, A.R., Tan, B.H., Loh, J.P., Barkham, T., Chow, V.T.K., Lin, R.T.P., Leo, Y.-S. (2010). Serological response in RT-PCR confirmed h1n1-2009 influenza a by hemagglutination inhibition and virus neutralization assays: An observational study. PLoS ONE 5 (8) : -. ScholarBank@NUS Repository.
Abstract: Background: We describe the serological response following H1N1-2009 influenza A infections confirmed by reversetranscriptase polymerase chain reaction (RT-PCR). Methodology and Principal Findings: The study included patients admitted to hospital, subjects of a seroepidemiologic cohort study, and participants identified from outbreak studies in Singapore. Baseline (first available blood sample) and follow-up blood samples were analyzed for antibody titers to H1N1-2009 and recently circulating seasonal influenza A virus strains by hemagglutination inhibition (HI) and virus micro-neutralization (VM) assays. 267 samples from 118 cases of H1N1- 2009 were analyzed. Geometric mean titers by HI peaked at 123 (95% confidence interval, CI 43-356) between days 30 to 39. The chance of observing seroconversion (four-fold or greater increase of antibodies) was maximized when restricting analysis to 45 participants with baseline sera collected within 5 days of onset and follow-up sera collected 15 or more days after onset; for these participants, 82% and 89% seroconverted to A/California/7/2009 H1N1 by HI and VM respectively. A four-fold or greater increase in cross-reactive antibody titers to seasonal A/Brisbane/59/2007 H1N1, A/Brisbane/10/2007 H3N2 and A/Wisconsin/15/2009 H3N2 occurred in 20%, 18% and 16% of participants respectively. Conclusions and Significance: Appropriately timed paired serology detects 80-90% RT-PCR confirmed H1N1-2009; Antibodies from infection with H1N1-2009 cross-reacted with seasonal influenza viruses. © 2010 Chen et al.
Source Title: PLoS ONE
ISSN: 19326203
DOI: 10.1371/journal.pone.0012474
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