Please use this identifier to cite or link to this item: https://doi.org/10.1002/chem.201103206
DC FieldValue
dc.titleCurrent chemical biology tools for studying protein phosphorylation and dephosphorylation
dc.contributor.authorH.s. Lu, C.
dc.contributor.authorLiu, K.
dc.contributor.authorTan, L.P.
dc.contributor.authorYao, S.Q.
dc.date.accessioned2014-05-19T02:51:00Z
dc.date.available2014-05-19T02:51:00Z
dc.date.issued2012-01-02
dc.identifier.citationH.s. Lu, C., Liu, K., Tan, L.P., Yao, S.Q. (2012-01-02). Current chemical biology tools for studying protein phosphorylation and dephosphorylation. Chemistry - A European Journal 18 (1) : 28-39. ScholarBank@NUS Repository. https://doi.org/10.1002/chem.201103206
dc.identifier.issn09476539
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/52857
dc.description.abstractAmongst different posttranslational events involved in cellular-signaling pathways, phosphorylation and dephosphorylation of proteins are the most prevalent. Aberrant regulations in the cellular phosphoproteome network are implicated in most major human diseases. Consequently, kinases and phosphatases are two of the most important groups of drug targets in medicinal research today. A major challenge in the understanding of protein phosphorylation and dephosphorylation is the sheer complexity of the phosphoproteome network and the lack of tools capable of studying protein phosphorylation and dephosphorylation as they occur in cells. We highlight herein various chemical biology tools that have emerged in the last decade for such studies. First, we discuss the use of small-molecule mimics of phosphoamino acids and their use in elucidating the function of protein phosphorylation and dephosphorylation. We also introduce recent advances in the field of activity-based protein profiling (ABPP) for proteome-wide detection of protein phosphorylation and dephosphorylation. We next discuss the key concepts in the design of peptide-and protein-based biosensors capable of real-time reporting of phosphorylation/dephosphorylation events. Finally, we highlight the application of peptide and small-molecule microarrays (SMMs), and their applications in high-throughput screening and discovery of new compounds related to phosphorylation/dephosphorylation. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/chem.201103206
dc.sourceScopus
dc.subjectactivity-based protein profiling
dc.subjectbiosensors
dc.subjectkinase
dc.subjectphosphatase
dc.subjectphosphorylation
dc.subjectsmall-molecule microarray
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1002/chem.201103206
dc.description.sourcetitleChemistry - A European Journal
dc.description.volume18
dc.description.issue1
dc.description.page28-39
dc.description.codenCEUJE
dc.identifier.isiut000298547800002
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

29
checked on Feb 10, 2020

WEB OF SCIENCETM
Citations

30
checked on Feb 10, 2020

Page view(s)

111
checked on Feb 4, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.