Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2006.11.018
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dc.titleQuantum-dot based nanoparticles for targeted silencing of HER2/neu gene via RNA interference
dc.contributor.authorTan, W.B.
dc.contributor.authorJiang, S.
dc.contributor.authorZhang, Y.
dc.date.accessioned2014-05-16T04:59:01Z
dc.date.available2014-05-16T04:59:01Z
dc.date.issued2007-03
dc.identifier.citationTan, W.B., Jiang, S., Zhang, Y. (2007-03). Quantum-dot based nanoparticles for targeted silencing of HER2/neu gene via RNA interference. Biomaterials 28 (8) : 1565-1571. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2006.11.018
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/52547
dc.description.abstractGene silencing using short interfering RNA (siRNA) is fast becoming an attractive approach to probe gene function in mammalian cells. Although there have been some success in the delivery of siRNA using various methods, tracking their delivery and monitoring their transfection efficiency prove to be hard without a suitable tracking agent. Therefore, a challenge lies with the design of an efficient and at the same time, self-tracking, transfection agent for RNA interference. In this paper, chitosan nanoparticles (NPs) with encapsulated quantum dots (QDs) were synthesized and used to deliver HER2/neu siRNA. Using such a construct, the delivery and transfection of the siRNA can be monitored by the presence of fluorescent QDs in the chitosan NPs. Targeted delivery of HER2 siRNA to HER2-overexpressing SKBR3 breast cancer cells was shown to be specific with chitosan/QD NP surface labeled with HER2 antibody targeting the HER2 receptors on SKBR3 cells. Gene-silencing effects of the conjugated siRNA was also established using the luciferase and HER2 ELISA assays. These self-tracking siRNA delivery NPs will also aid in the monitoring of future gene silencing studies in vivo. © 2006 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2006.11.018
dc.sourceScopus
dc.subjectChitosan
dc.subjectFluorescence
dc.subjectGene therapy
dc.subjectNanoparticle
dc.typeArticle
dc.contributor.departmentBIOENGINEERING
dc.contributor.departmentNUS NANOSCIENCE & NANOTECH INITIATIVE
dc.description.doi10.1016/j.biomaterials.2006.11.018
dc.description.sourcetitleBiomaterials
dc.description.volume28
dc.description.issue8
dc.description.page1565-1571
dc.description.codenBIMAD
dc.identifier.isiut000244130000012
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