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Title: Stability and hybridization-driven aggregation of silver nanoparticle-oligonucleotide conjugates
Authors: Vidal Jr., B.C. 
Deivaraj, T.C. 
Yang, J.
Too, H.-P.
Chow, G.-M. 
Gan, L.M.
Lee, J.Y. 
Issue Date: Jun-2005
Citation: Vidal Jr., B.C., Deivaraj, T.C., Yang, J., Too, H.-P., Chow, G.-M., Gan, L.M., Lee, J.Y. (2005-06). Stability and hybridization-driven aggregation of silver nanoparticle-oligonucleotide conjugates. New Journal of Chemistry 29 (6) : 812-816. ScholarBank@NUS Repository.
Abstract: Gold nanoparticles are well known to form stable oligonucleotide conjugates, via thiol-metal interaction, which are capable of specific DNA-hybridization induced aggregation. Preparation of an analogous conjugate with Ag particles, except when coated with a layer of Au, has been reported to yield conjugates that are unstable in the hybridization environment. We report herein the hybridization-induced aggregation of such "bare" Ag-particle conjugates prepared via a similar facile procedure to Au with slight modification. We found that the pH during functionalization is the critical factor determining the success of preparing conjugates that remain stable throughout the functionalization process and during hybridization. We reasoned that this is a consequence of the pH-dependent charge of the oligonucleotide, and demonstrated that pH strongly affects the amount of oligonucleotides adsorbed on the particle surface, thereby imparting stability to the particles. This finding has the potential to be generalized to other metal particle-oligonucleotide systems with borderline stability, helping to expand the repertoire of visible-range plasmon signatures useful for diagnostic application. © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2005.
Source Title: New Journal of Chemistry
ISSN: 11440546
DOI: 10.1039/b417683a
Appears in Collections:Staff Publications

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