Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.febslet.2007.05.020
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dc.titleCollagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: The biological relevance of the excluded volume effect
dc.contributor.authorLareu, R.R.
dc.contributor.authorSubramhanya, K.H.
dc.contributor.authorPeng, Y.
dc.contributor.authorBenny, P.
dc.contributor.authorChen, C.
dc.contributor.authorWang, Z.
dc.contributor.authorRajagopalan, R.
dc.contributor.authorRaghunath, M.
dc.date.accessioned2014-04-25T09:03:47Z
dc.date.available2014-04-25T09:03:47Z
dc.date.issued2007-06-12
dc.identifier.citationLareu, R.R., Subramhanya, K.H., Peng, Y., Benny, P., Chen, C., Wang, Z., Rajagopalan, R., Raghunath, M. (2007-06-12). Collagen matrix deposition is dramatically enhanced in vitro when crowded with charged macromolecules: The biological relevance of the excluded volume effect. FEBS Letters 581 (14) : 2709-2714. ScholarBank@NUS Repository. https://doi.org/10.1016/j.febslet.2007.05.020
dc.identifier.issn00145793
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/51764
dc.description.abstractThe excluded volume effect (EVE) rules all life processes. It is created by macromolecules that occupy a given volume thereby confining other molecules to the remaining space with large consequences on reaction kinetics and molecular assembly. Implementing EVE in fibroblast culture accelerated conversion of procollagen to collagen by procollagen C-proteinase (PCP/BMP-1) and proteolytic modification of its allosteric regulator, PCOLCE1. This led to a 20-30- and 3-6-fold increased collagen deposition in two- and three-dimensional cultures, respectively, and creation of crosslinked collagen footprints beneath cells. Important parameters correlating with accelerated deposition were hydrodynamic radius of macromolecules and their negative charge density. © 2007.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.febslet.2007.05.020
dc.sourceScopus
dc.subjectCollagen deposition
dc.subjectExcluded volume effect
dc.subjectExtracellular matrix
dc.subjectMacromolecular crowding
dc.subjectProcollagen C-proteinase
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.1016/j.febslet.2007.05.020
dc.description.sourcetitleFEBS Letters
dc.description.volume581
dc.description.issue14
dc.description.page2709-2714
dc.description.codenFEBLA
dc.identifier.isiut000247324300023
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