The effect of Rhbmp-2 on canine osteoblasts seeded onto 3D bioactive polycaprolactone scaffolds

Abstract

The effect of human recombinant bone morphogenetic protein-2 (rhBMP-2) on canine osteoblasts seeded onto three dimensional bioactive polycaprolactone 20% tricalcium phosphate (PCL-TCP) scaffolds was investigated. Canine osteoblasts, rhBMP-2 of various concentrations and fibrin glue were simultaneously seeded onto the top of the porous scaffolds which were placed in 24-well culture plates. It was observed that rhBMP-2 enhanced the differentiated function of canine osteoblasts in a non-dose dependent manner, which resulted in accelerated mineralization, followed by death of osteoblasts as they underwent terminal differentiation. PCL-TCP scaffolds seeded only with canine osteoblasts was found to sustain excellent osteogenic expression in vitro.