Please use this identifier to cite or link to this item:
Title: A dual-functional fibrous scaffold enhances P450 activity of cultured primary rat hepatocytes
Authors: Chua, K.-N.
Tang, Y.-N.
Quek, C.-H.
Ramakrishna, S. 
Leong, K.W.
Mao, H.-Q.
Keywords: Drug encapsulation
Electrospun fiber
Hepatocyte culture
Surface modification
Issue Date: Sep-2007
Citation: Chua, K.-N., Tang, Y.-N., Quek, C.-H., Ramakrishna, S., Leong, K.W., Mao, H.-Q. (2007-09). A dual-functional fibrous scaffold enhances P450 activity of cultured primary rat hepatocytes. Acta Biomaterialia 3 (5) : 643-650. ScholarBank@NUS Repository.
Abstract: We have designed a novel dual-functional electrospun fibrous scaffold comprising two fiber mesh layers that were modified differently to induce two separate biological responses from hepatocytes. The first fiber layer was galactosylated on the surface to mediate hepatocyte attachment, while the second layer was loaded with 3-methylcholanthrene (3-Mc) to enhance cytochrome P450 activity of hepatocytes. Primary rat hepatocytes cultured on the galactosylated fibrous scaffolds loaded with different concentrations of 3-Mc were compared for their cell attachment efficiency, albumin secretion activity and cytochrome P450-dependent 7-ethoxycoumarin O-deethylase activity. This hybrid fibrous scaffold mediated hepatocyte attachment with slightly lower efficiency (76 ± 2.3%) than a single-layer galactosylated fibrous scaffold (84 ± 3.5%). More importantly, the cytochrome P450 activity of the hepatocytes cultured on the hybrid scaffold correlated well with the 3-Mc loading level. The results also showed that transfer of 3-Mc to hepatocytes through direct cell-fiber contact was the dominant transport route, with the induced cytochrome P450 activity being 1.9- to 4.8-fold higher than that of transfer of 3-Mc to hepatocytes via dissolution from fibers to medium. This study demonstrates the feasibility of creating multi-functional fibrous scaffolds that serve both as an adhesive substrate and as a delivery vehicle for bioactive molecules. © 2007 Acta Materialia Inc.
Source Title: Acta Biomaterialia
ISSN: 17427061
DOI: 10.1016/j.actbio.2007.03.012
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on May 27, 2020


checked on May 18, 2020

Page view(s)

checked on May 12, 2020

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.