Please use this identifier to cite or link to this item: https://doi.org/10.1002/dvdy.10273
DC FieldValue
dc.titleRecapitulation of fast skeletal muscle development in zebrafish by transgenic expression of GFP under the mylz2 promoter
dc.contributor.authorJu, B.
dc.contributor.authorChong, S.W.
dc.contributor.authorHe, J.
dc.contributor.authorWang, X.
dc.contributor.authorXu, Y.
dc.contributor.authorWan, H.
dc.contributor.authorTong, Y.
dc.contributor.authorYan, T.
dc.contributor.authorKorzh, V.
dc.contributor.authorGong, Z.
dc.date.accessioned2014-04-23T07:09:01Z
dc.date.available2014-04-23T07:09:01Z
dc.date.issued2003-05-01
dc.identifier.citationJu, B., Chong, S.W., He, J., Wang, X., Xu, Y., Wan, H., Tong, Y., Yan, T., Korzh, V., Gong, Z. (2003-05-01). Recapitulation of fast skeletal muscle development in zebrafish by transgenic expression of GFP under the mylz2 promoter. Developmental Dynamics 227 (1) : 14-26. ScholarBank@NUS Repository. https://doi.org/10.1002/dvdy.10273
dc.identifier.issn10588388
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/50726
dc.description.abstractA 1,934-bp muscle-specific promoter from the zebrafish mylz2 gene was isolated and characterized by transgenic analysis. By using a series of 5′ promoter deletions linked to the green fluorescent protein (gfp) reporter gene, transient transgenic analysis indicated that the strength of promoter activity appeared to correlate to the number of muscle cis-elements in the promoter and that a minimal -77-bp region was sufficient for a relatively strong promoter activity in muscle cells. Stable transgenic lines were obtained from several mylz2-gfp constructs. GFP expression in the 1,934-bp promoter transgenic lines mimicked well the expression pattern of endogenous mylz2 mRNA in both somitic muscle and nonsomitic muscles, including fin, eye, jaw, and gill muscles. An identical pattern of GFP expression, although at a much lower level, was observed from a transgenic line with a shorter 871-bp promoter. Our observation indicates that there is no distinct cis-element for activation of mylz2 in different skeletal muscles. Furthermore, RNA encoding a dominant negative form of cAMP-dependent protein kinase A was injected into mylz2-gfp transgenic embryos and GFP expression was significantly reduced due to an expanded slow muscle development at the expense of GFP-expressing fast muscle. The mylz2-gfp transgene was also transferred into two zebrafish mutants, spadetail and chordino, and several novel phenotypes in muscle development in these mutants were discovered. © 2003 Wiley-Liss, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/dvdy.10273
dc.sourceScopus
dc.subjectcAMP-dependent protein kinase A (PKA)
dc.subjectChordino (din)
dc.subjectE-box
dc.subjectEnhanced green fluorescent protein (EGFP)
dc.subjectMEF2
dc.subjectMyosin light chain
dc.subjectPromoter
dc.subjectSpadetail (spt)
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentINSTITUTE OF MOLECULAR AGROBIOLOGY
dc.contributor.departmentCIVIL ENGINEERING
dc.description.doi10.1002/dvdy.10273
dc.description.sourcetitleDevelopmental Dynamics
dc.description.volume227
dc.description.issue1
dc.description.page14-26
dc.description.codenDEDYE
dc.identifier.isiut000182571300002
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

54
checked on Dec 9, 2019

WEB OF SCIENCETM
Citations

51
checked on Dec 9, 2019

Page view(s)

78
checked on Dec 2, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.