Please use this identifier to cite or link to this item:
|Title:||Evidence of autoantibodies to glutamic acid decarboxylase in oral fluid of type 1 diabetic patients||Authors:||Todd, A.L.
|Keywords:||Gingival crevicular fluid
|Issue Date:||2002||Citation:||Todd, A.L., Ng, W.-Y., Thai, A.C., Lee, Y.S., Loke, K.Y. (2002). Evidence of autoantibodies to glutamic acid decarboxylase in oral fluid of type 1 diabetic patients. Diabetes Research and Clinical Practice 57 (3) : 171-177. ScholarBank@NUS Repository. https://doi.org/10.1016/S0168-8227(02)00059-1||Abstract:||Circulating antibodies to glutamic acid decarboxylase (GADab) are a major indicator for autoimmune destruction of pancreatic islet cells (type 1 diabetes). Previously reported detection of GADab in oral fluid, however, was assayed by enzyme-linked immunosorbent assay (ELISA) with low diagnostic sensitivity and high non-specific binding. We re-assessed oral fluid GADab detection using a different sampling technique and a more robust assay. Type 1 diabetic subjects (n=32; mean age±SD: 13.9±3.7 years) provided Orasure® oral fluid and venous blood samples. Orasure® collections were assayed for total immunoglobulin G (IgG), then concentrated to 1/10 of their volume using mini-centrifugal protein concentrators. All samples were assayed by a GAD65 antibody radio-immunoprecipitation method. Oral fluid antibodies were detected (>99th percentile of radio-binding (%counts per min (%cpm)) for seronegatives) in 10/16 seropositive subjects, with %cpm (median: 6.4%; range: 4.6-25.8) significantly greater (P<0.001) than for seronegatives (median: 4.7%; range: 3.4-5.7). A highly significant correlation (Spearman's rho: 0.85; P<0.001) was demonstrated between %cpm of concentrates and respective serum titres for seropositive diabetics. Median IgG concentration of Orasure® collections was 22.8 mg/l (range: 9.4-168.0). GADab recovery from Orasure® collectors was estimated at 90%. This is the first confirmatory detection of diabetes-specific autoimmune markers in oral fluid. Acceptable correlation between concentrated oral fluid radio-binding and serum titre was achieved. Improved antibody recovery and assay re-optimisation could provide a basis for more extensive studies that may lead to an alternative non-invasive screening method for pre-clinical autoimmune diabetes. © 2002 Elsevier Science Ireland Ltd. All rights reserved.||Source Title:||Diabetes Research and Clinical Practice||URI:||http://scholarbank.nus.edu.sg/handle/10635/48172||ISSN:||01688227||DOI:||10.1016/S0168-8227(02)00059-1|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Aug 16, 2019
WEB OF SCIENCETM
checked on Aug 16, 2019
checked on Aug 18, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.