Please use this identifier to cite or link to this item: https://doi.org/10.1111/j.1600-0765.2012.01519.x
DC FieldValue
dc.titleGlycated matrix up-regulates inflammatory signaling similarly to Porphyromonas gingivalis lipopolysaccharide
dc.contributor.authorChang, P.-C.
dc.contributor.authorChien, L.-Y.
dc.contributor.authorChong, L.Y.
dc.contributor.authorKuo, Y.-P.
dc.contributor.authorHsiao, J..-K.
dc.date.accessioned2013-10-16T07:24:37Z
dc.date.available2013-10-16T07:24:37Z
dc.date.issued2013
dc.identifier.citationChang, P.-C., Chien, L.-Y., Chong, L.Y., Kuo, Y.-P., Hsiao, J..-K. (2013). Glycated matrix up-regulates inflammatory signaling similarly to Porphyromonas gingivalis lipopolysaccharide. Journal of Periodontal Research 48 (2) : 184-193. ScholarBank@NUS Repository. https://doi.org/10.1111/j.1600-0765.2012.01519.x
dc.identifier.issn00223484
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/47167
dc.description.abstractBackground and Objective: Hyperglycemia and advanced glycation end-products (AGEs) have been hypothesized as the etiologic factors of diabetic periodontitis. The aim of this study was to clarify in greater detail the patterns of AGE-mediated periodontal inflammation under various physiological conditions. Material and Methods: The deposition of AGEs and expression of the receptor for AGEs (RAGE) were identified by immunohistochemistry in Sprague-Dawley rats with experimentally induced periodontitis or diabetes. Human periodontal ligament cells (PDLCs) and mesenchymal stem cells (MSCs) were cultured under simulated conditions of hyperglycemia, Porphyromonas gingivalis lipopolysaccharide (LPS) stimulation and matrix glycation. Cell viability and expression of toll-like receptors (TLRs), Rage, an inflammatory signaling initiator (nuclear factor kappa light chain enhancer of activator β cells), an oxidative stressor (heme oxygenase-1) and collagen synthesis (type I and type IV) genes were evaluated. Results: The deposition of AGEs and the expression of Rage were evident in the inflamed periodontal tissues in all rats and appeared to be enhanced in rats with diabetes. Matrix glycation augmented cytotoxicity, up-regulated RAGE and TLRs in both PDLCs and MSCs, and significantly activated downstream inflammatory signaling in MSCs. Oxidative stress was significantly increased under matrix glycation in both PDLCs and MSCs and was significantly increased at a high-glucose concentration in MSCs. A consistent decrease in expression of type I and type IV collagens was observed in MSCs, but a delayed reduction was noted in PDLCs. Conclusions: Matrix glycation modulated cell behavior to induce inflammation equivalent to that produced by incubation with P. gingivalis LPS. Periodontal inflammation also led to matrix glycation, thus demonstrating a definite interaction between diabetes and periodontitis. © 2012 John Wiley & Sons A/S.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1111/j.1600-0765.2012.01519.x
dc.sourceScopus
dc.subjectAdvanced
dc.subjectDiabetes
dc.subjectGlycosylation end-products
dc.subjectin-vitro model
dc.subjectPeriodontal disease
dc.typeArticle
dc.contributor.departmentDENTISTRY
dc.description.doi10.1111/j.1600-0765.2012.01519.x
dc.description.sourcetitleJournal of Periodontal Research
dc.description.volume48
dc.description.issue2
dc.description.page184-193
dc.description.codenJPDRA
dc.identifier.isiut000315469600007
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