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|Title:||The effect of cyclic mechanical strain on the expression of adhesion-related genes by periodontal ligament cells in two-dimensional culture||Authors:||Saminathan, A.
Cyclic mechanical strain
Periodontal ligament cell
Real-time RT-PCR microarray
|Issue Date:||2012||Citation:||Saminathan, A., Vinoth, K.J., Wescott, D.C., Pinkerton, M.N., Milne, T.J., Cao, T., Meikle, M.C. (2012). The effect of cyclic mechanical strain on the expression of adhesion-related genes by periodontal ligament cells in two-dimensional culture. Journal of Periodontal Research 47 (2) : 212-221. ScholarBank@NUS Repository. https://doi.org/10.1111/j.1600-0765.2011.01423.x||Abstract:||Background and Objective: Cell adhesion plays important roles in maintaining the structural integrity of connective tissues and sensing changes in the biomechanical environment of cells. The objective of the present investigation was to extend our understanding of the effect of cyclic mechanical strain on the expression of adhesion-related genes by human periodontal ligament cells. Material and Methods: Cultured periodontal ligament cells were subjected to a cyclic in-plane tensile deformation of 12% for 5s (0.2Hz) every 90s for 6-24h in a Flexercell FX-4000 Strain Unit. The following parameters were measured: (i)cell viability by the MTT assay; (ii)caspase-3 and -7 activity; and (iii)the expression of 84 genes encoding adhesion-related molecules using real-time RT-PCR microarrays. Results: Mechanical stress reduced the metabolic activity of deformed cells at 6h, and caspase-3 and -7 activity at 6 and 12h. Seventy-three genes were detected at critical threshold values <35. Fifteen showed a significant change in relative expression: five cell adhesion molecules (ICAM1, ITGA3, ITGA6, ITGA8 and NCAM1), three collagen α-chains (COL6A1, COL8A1 and COL11A1), four MMPs (ADAMTS1, MMP8, MMP11 and MMP15), plus CTGF, SPP1 and VTN. Four genes were upregulated (ADAMTS1, CTGF, ICAM1 and SPP1) and 11 downregulated, with the range extending from a 1.76-fold induction of SPP1 at 12h to a 2.49-fold downregulation of COL11A1 at 24h. Conclusion: The study has identified several mechanoresponsive adhesion-related genes, and shown that onset of mechanical stress was followed by a transient reduction in overall cellular activity, including the expression of two apoptosis 'executioner' caspases. © 2011 John Wiley & Sons A/S.||Source Title:||Journal of Periodontal Research||URI:||http://scholarbank.nus.edu.sg/handle/10635/47143||ISSN:||00223484||DOI:||10.1111/j.1600-0765.2011.01423.x|
|Appears in Collections:||Staff Publications|
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