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dc.titleChairside sensor for rapid monitoring of Enterococcus faecalis activity
dc.contributor.authorKishen, A.
dc.contributor.authorChen, N.N.
dc.contributor.authorTan, L.
dc.contributor.authorAsundi, A.
dc.identifier.citationKishen, A.,Chen, N.N.,Tan, L.,Asundi, A. (2004). Chairside sensor for rapid monitoring of Enterococcus faecalis activity. Journal of Endodontics 30 (12) : 872-875. ScholarBank@NUS Repository.
dc.description.abstractIn this study, optical spectroscopy was used to monitor a chromogenic, enzyme-substrate reaction for the rapid identification of Enterococcus faecalis. The detection system, comprising a miniature spectrophotometer and an accompanying data acquisition system, was placed in an incubator. During testing, a 3-ml test sample was placed in a cuvette within the spectrophotometer. This permitted online, real-time, and remote analysis of spectral signature needed to monitor the bacteria. It was observed that the absorption peak intensity increased conspicuously 3.5 h after inoculation and through the entire period of testing. A linear-regression analysis demonstrated a significant correlation between the increase in absorption peak intensity at 610 nm (r = 0.9389) and 653 nm (r = 0.9387) with the formation of colony-forming units. Optical spectroscopy-based sensing systems can pave the way for rapid, nonlaboratory-based approaches to monitor microbial status quantitatively and qualitatively from clinical samples.
dc.contributor.departmentRESTORATIVE DENTISTRY
dc.description.sourcetitleJournal of Endodontics
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