Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/46816
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dc.titleChairside sensor for rapid monitoring of Enterococcus faecalis activity
dc.contributor.authorKishen, A.
dc.contributor.authorChen, N.N.
dc.contributor.authorTan, L.
dc.contributor.authorAsundi, A.
dc.date.accessioned2013-10-16T05:50:27Z
dc.date.available2013-10-16T05:50:27Z
dc.date.issued2004
dc.identifier.citationKishen, A.,Chen, N.N.,Tan, L.,Asundi, A. (2004). Chairside sensor for rapid monitoring of Enterococcus faecalis activity. Journal of Endodontics 30 (12) : 872-875. ScholarBank@NUS Repository.
dc.identifier.issn00992399
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/46816
dc.description.abstractIn this study, optical spectroscopy was used to monitor a chromogenic, enzyme-substrate reaction for the rapid identification of Enterococcus faecalis. The detection system, comprising a miniature spectrophotometer and an accompanying data acquisition system, was placed in an incubator. During testing, a 3-ml test sample was placed in a cuvette within the spectrophotometer. This permitted online, real-time, and remote analysis of spectral signature needed to monitor the bacteria. It was observed that the absorption peak intensity increased conspicuously 3.5 h after inoculation and through the entire period of testing. A linear-regression analysis demonstrated a significant correlation between the increase in absorption peak intensity at 610 nm (r = 0.9389) and 653 nm (r = 0.9387) with the formation of colony-forming units. Optical spectroscopy-based sensing systems can pave the way for rapid, nonlaboratory-based approaches to monitor microbial status quantitatively and qualitatively from clinical samples.
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentRESTORATIVE DENTISTRY
dc.description.sourcetitleJournal of Endodontics
dc.description.volume30
dc.description.issue12
dc.description.page872-875
dc.description.codenJOEND
dc.identifier.isiutNOT_IN_WOS
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