Please use this identifier to cite or link to this item:
Title: Inorganic phosphate is the major component of the thermostable cytoplasmic fraction which stimulates mitochondrial anion uniport
Authors: Ng, L.T. 
Selwyn, M.J. 
Choo, H.L.
Keywords: anion channel
inner membrane
inorganic phosphate
Issue Date: 1993
Citation: Ng, L.T., Selwyn, M.J., Choo, H.L. (1993). Inorganic phosphate is the major component of the thermostable cytoplasmic fraction which stimulates mitochondrial anion uniport. Biochimica et Biophysica Acta - Bioenergetics 1183 (1) : 180-184. ScholarBank@NUS Repository.
Abstract: A low molecular weight thermostable cytoplasmic fraction isolated from rat liver homogenate when pre-incubated with mitochondria increases the rate at which anions enter mitochondria via the pH-dependent anion-conducting channel in the inner membrane. The crude fraction obtained by centrifuging and heating the liver homogenate was purified by gel filtration and chromatography on DEAE-cellulose. The resulting factor is stable to heating at 100°C, freeze-drying and extremes of pH. Inorganic phosphate co-purified with activity and activity was lost when the phosphate was removed by barium salt precipitation. A pure sample of KH2PO4 produced stimulation of anion conductivity. These results show that the major portion of the activity which stimulates anion uniport can be accounted for by the presence of phosphate in the crude and purified fractions. Mersalyl blocks stimulation when added before, but not when added after, incubation with phosphate which shows that the stimulation is produced by phosphate in the mitochondrial matrix. The proposed role of this factor in thyroid hormone action is discussed in the light of its identification as inorganic phosphate.
Source Title: Biochimica et Biophysica Acta - Bioenergetics
ISSN: 00052728
DOI: 10.1016/0005-2728(93)90016-9
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Apr 17, 2019


checked on Apr 10, 2019

Page view(s)

checked on Apr 11, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.