Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0167-4781(96)00209-6
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dc.titleCloning, characterization and expression of a cDNA clone encoding rabbit ubiquitin-conjugating enzyme, E2(32k)
dc.contributor.authorSun, B.
dc.contributor.authorJeyaseelan, K.
dc.contributor.authorChung, M.C.M.
dc.contributor.authorTian-Seng, T.
dc.contributor.authorTan, T.-W.
dc.contributor.authorChock, P.B.
dc.date.accessioned2013-06-05T09:49:50Z
dc.date.available2013-06-05T09:49:50Z
dc.date.issued1997
dc.identifier.citationSun, B., Jeyaseelan, K., Chung, M.C.M., Tian-Seng, T., Tan, T.-W., Chock, P.B. (1997). Cloning, characterization and expression of a cDNA clone encoding rabbit ubiquitin-conjugating enzyme, E2(32k). Biochimica et Biophysica Acta - Gene Structure and Expression 1351 (1-2) : 231-238. ScholarBank@NUS Repository. https://doi.org/10.1016/S0167-4781(96)00209-6
dc.identifier.issn01674781
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/38283
dc.description.abstractA cDNA clone encoding rabbit E2(32k) was obtained by library screening and PCR. The cDNA contains an open reading frame coding for 238 amino acids which shows an overall identity of 81% to human CDC34, the cell cycle-related ubiquitin-conjugating enzyme. A 50% homology to yeast CDC34 within the conserved core domain was also observed. Northern blot analysis indicated that three transcripts existed in all six rabbit tissues examined but their expression levels varied over a wide range. The putative cDNA coding region was highly expressed in Escherichia coli as a his-tagged protein which was purified to homogeneity. The ability of this expressed protein to form a thiolester bond with ubiquitin showed that it was functionally active, The ability of this protein to catalyze the conjugation of ubiquitin to histone H2A and H2B was also examined.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0167-4781(96)00209-6
dc.sourceScopus
dc.subjectCloning
dc.subjectE2(32k)
dc.subjectProtein degradation
dc.subjectRabbit
dc.subjectUbiquitin-conjugating enzyme
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/S0167-4781(96)00209-6
dc.description.sourcetitleBiochimica et Biophysica Acta - Gene Structure and Expression
dc.description.volume1351
dc.description.issue1-2
dc.description.page231-238
dc.description.codenBBGSD
dc.identifier.isiutA1997WP45100026
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