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Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0378-1119(97)00154-6
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dc.titleRabbit ubiquitin-activating enzyme E1: cDNA cloning, sequence and expression
dc.contributor.authorSun, B.
dc.contributor.authorJeyaseelan, K.
dc.contributor.authorChung, M.C.M.
dc.contributor.authorTeo, T.-S.
dc.date.accessioned2013-06-05T09:47:01Z
dc.date.available2013-06-05T09:47:01Z
dc.date.issued1997
dc.identifier.citationSun, B., Jeyaseelan, K., Chung, M.C.M., Teo, T.-S. (1997). Rabbit ubiquitin-activating enzyme E1: cDNA cloning, sequence and expression. Gene 196 (1-2) : 19-23. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-1119(97)00154-6
dc.identifier.issn03781119
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/38154
dc.description.abstractA cDNA clone encoding ubiquitin-activating enzyme E1 has been isolated from a rabbit heart cDNA library and sequenced. The 3.485 kb cDNA contains an open reading frame of 1058 amino acid residues which predicts a protein of approx. 118 kDa. The deduced protein sequence exhibits a very high homology to other ubiquitin-activating enzymes identified in a variety of organisms. Northern blot analysis reveals a single transcript of apporx. 3.5 kb in all the rabbit tissues examined. The entire coding region of the rabbit E1 cDNA has been expressed as a his-tagged protein. The recombinant protein has been verified by its ability to cross-react with anti-human E1 antibodies. Ubiquitin thiolester assay shows that the recombinant rabbit E1 protein is functional.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0378-1119(97)00154-6
dc.sourceScopus
dc.subjectProtein degradation
dc.subjectTissue distribution
dc.subjectUbiquitination
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/S0378-1119(97)00154-6
dc.description.sourcetitleGene
dc.description.volume196
dc.description.issue1-2
dc.description.page19-23
dc.description.codenGENED
dc.identifier.isiutA1997XV96200003
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