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Title: α-neurotoxin gene expression in Naja sputatrix: Identification of a silencer element in the promoter region
Authors: Ma, D.
Armugam, A. 
Jeyaseelan, K. 
Keywords: Cardiotoxin-2
Naja sputatrix
Issue Date: 2002
Citation: Ma, D., Armugam, A., Jeyaseelan, K. (2002). α-neurotoxin gene expression in Naja sputatrix: Identification of a silencer element in the promoter region. Archives of Biochemistry and Biophysics 404 (1) : 98-105. ScholarBank@NUS Repository.
Abstract: Alpha-neurotoxin (α-NTX) from the venom of cobra, Naja sputatrix, is a highly lethal post-synaptic toxin that is responsible for the lethality caused by the venom. However, this toxin is found at low levels (3%) in the crude venom. The expression of its gene is determined by a promoter which is 90% similar to the promoter of another three-fingered toxin, cardiotoxin (CTX), which is produced in large amounts (60%) in the same venom. Functional analysis of the NTX-2 gene promoter demonstrated the presence of a silencer element of 24 nucleotides (nt -678 to -655) at its 5′ flanking region. This element has been found to play a major role in the down-regulation of NTX-2 gene expression. A point mutation on this silencer appears to attenuate its repressive property in CTX-2 gene. © 2002 Elsevier Science (USA). All rights reserved.
Source Title: Archives of Biochemistry and Biophysics
ISSN: 00039861
DOI: 10.1016/S0003-9861(02)00245-X
Appears in Collections:Staff Publications

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