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https://scholarbank.nus.edu.sg/handle/10635/35444
Title: | METHODS OF ASSAYING DNA-REPAIRING ENZYMES AND THEIR ALKYLATED DERIVATIVES | Authors: | LI, BENJAMIN FUK LOI AYI, TECK CHOON |
Issue Date: | 20-Jul-1995 | Citation: | LI, BENJAMIN FUK LOI,AYI, TECK CHOON (1995-07-20). METHODS OF ASSAYING DNA-REPAIRING ENZYMES AND THEIR ALKYLATED DERIVATIVES. ScholarBank@NUS Repository. | Abstract: | The present invention provides methods of assaying the human DNA repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) and/or active site alkylated derivatives thereof (R-MGMT), which rely on the ability of a protease, e.g. V8 Protease (also referred to as Glu-C Protease), to distinguish between these two enzyme forms. Such a method comprises the steps of: (a) contacting the sample with a protease to which one of human MGMT and R-MGMT is preferentially sensitive; and (b) determining whether the treated sample contains one or more polypeptides selected from (i) at least one polypeptide fragment characteristic of action of the protease on the protease-sensitive form of human MGMT and (ii) the protease-resistant form of human MGMT. | URI: | http://scholarbank.nus.edu.sg/handle/10635/35444 |
Appears in Collections: | Staff Publications |
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