Please use this identifier to cite or link to this item:
|Title:||Involvement of the L-arginine-nitric oxide synthase pathway in the relaxant responses of the rat isolated anococcygeus muscle to a scorpion (Leiurus quinquestriatus quinquestriatus) venom||Authors:||Gwee, M.C.E.
|Issue Date:||1995||Citation:||Gwee, M.C.E., Cheah, L.S., Gopalakrishnakone, P. (1995). Involvement of the L-arginine-nitric oxide synthase pathway in the relaxant responses of the rat isolated anococcygeus muscle to a scorpion (Leiurus quinquestriatus quinquestriatus) venom. Toxicon 33 (9) : 1141-1150. ScholarBank@NUS Repository. https://doi.org/10.1016/0041-0101(95)00064-S||Abstract:||Venom from several species of scorpions can cause generalized depolarization of peripheral nerves with enhancement of neurotransmitter release. The effects of the venom (LQV) from the scorpion Leiurus quinquestriatus quinquestriatus were investigated using the rat isolated carbachol (CCh) precontracted anococcygeus muscle (Acm) mounted in Krebs solution containing phentolamine (5 μM). LQV (0.2 μg/ml and 1.5 μg/ml) markedly relaxed the tone of the CCh precontracted (non-stimulated) Acm by 31.5 ± 5.1% and 35.5 ± 4.7%, respectively; the onset was immediate after the high dose, followed by a slow and gradual return of the muscle tone to 88.7 ± 1.8% of the initial peak tension in 50.2 ± 4 min. Subsequent doses of LQV produced essentially no appreciable changes in muscle tone, but the addition of L-arginine (250 μM) or, especially, sodium nitroprusside (SNP; 1 μM) produced marked and rapid relaxations of the Acm. Similar results were obtained with LQV during electrical field stimulation (EFS) of the precontracted Acm; however, during the gradual return of the muscle tone from the relaxed state induced by LQV, the EFS-induced relaxant (NANC) responses were also progressively inhibited by 84.9 ± 3.4% and 64.3 ± 4.5 by LQV 0.2 μg/ml and l.5 μg/ml, respectively. Tetrodotoxin (TTx; 2 μM) or N(G)-nitro-L-arginine methylester (L-NAME; 50 μM) markedly inhibited the relaxant responses of the Acm to EFS as well as to LQV but not the responses to SNP: L-arginine (250 μM) partially restored the relaxant (NANC) responses of the Acm to EFS. Thus, the L-arginine-nitric oxide synthase-nitric oxide pathway is involved in mediating the marked relaxant responses of the CCh precontracted Acm to LQV.||Source Title:||Toxicon||URI:||http://scholarbank.nus.edu.sg/handle/10635/33894||ISSN:||00410101||DOI:||10.1016/0041-0101(95)00064-S|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jan 17, 2020
WEB OF SCIENCETM
checked on Jan 10, 2020
checked on Dec 31, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.