Please use this identifier to cite or link to this item: https://doi.org/10.1016/0040-8166(90)90044-A
DC FieldValue
dc.titleAntiport-mediated cell retraction: Viable rounding and distinctive endocytosis
dc.contributor.authorSit, K.H.
dc.contributor.authorBay, B.H.
dc.contributor.authorWong, K.P.
dc.date.accessioned2012-06-08T09:29:27Z
dc.date.available2012-06-08T09:29:27Z
dc.date.issued1990
dc.identifier.citationSit, K.H., Bay, B.H., Wong, K.P. (1990). Antiport-mediated cell retraction: Viable rounding and distinctive endocytosis. Tissue and Cell 22 (6) : 785-802. ScholarBank@NUS Repository. https://doi.org/10.1016/0040-8166(90)90044-A
dc.identifier.issn00408166
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/33809
dc.description.abstractCells that are detached through Na+/H+ antiport-mediated cell retraction (the flat-to-round or FTR change) are as viable as trypsinized cells despite unusually high trypan blue dye inclusion. Even high molecular weight dextran and carbon particles are easily loaded following the FTR change. ECM of confluent cultures of human keloid fibroblasts and Chang liver epitheloid cells stained directly by fluorescein-conjugated monoclonal anti-fibronectin is dramatically reduced or removed upon cell rounding. At the ultrastructural level, distinctive channels and vacuoles filled with extracellular material are seen, resolved at later stages as peripheral granular patches which too disappear when the rounded cells are reflattened in round-to-flat (RTF) change. Antiport-mediated endocytosis (AME) could explain the drastic reduction in cell area concomitant with cell rounding and detachment. AME provides a new way of loading cells with impermeant macromolecules.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/0040-8166(90)90044-A
dc.sourceScopus
dc.subjectCell detachment
dc.subjectECM withdrawal
dc.subjectEndocytosis
dc.subjectMacromolecular loading
dc.subjectNa+/H+antiport
dc.typeArticle
dc.contributor.departmentANATOMY
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/0040-8166(90)90044-A
dc.description.sourcetitleTissue and Cell
dc.description.volume22
dc.description.issue6
dc.description.page785-802
dc.description.codenTICEB
dc.identifier.isiutA1990ER38700003
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

24
checked on Aug 14, 2019

WEB OF SCIENCETM
Citations

21
checked on Aug 14, 2019

Page view(s)

121
checked on Aug 19, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.