Please use this identifier to cite or link to this item:
|Title:||A major lethal factor of the venom of Burmese Russel's viper (Daboia russelli siamensis); Isolation, N-terminal sequencing and biological activities of daboiatoxin||Authors:||Maung-Maung-Thwin
|Issue Date:||1995||Citation:||Maung-Maung-Thwin, Gopalakrishnakone, P., Yuen, R., Tan, C.H. (1995). A major lethal factor of the venom of Burmese Russel's viper (Daboia russelli siamensis); Isolation, N-terminal sequencing and biological activities of daboiatoxin. Toxicon 33 (1) : 63-76. ScholarBank@NUS Repository. https://doi.org/10.1016/0041-0101(94)00133-S||Abstract:||A major lethal factor, daboiatoxin (DbTx), showing strong PLA2 activity (specific activity 91.7 nmoles/min/mg), was purified to homogeneity from the venom of Burmese Russell's viper (Daboia r. siamensis) by a combination of gel filtration on Sephadex G-75 and ion-exchange chromatography on CM-Sephadex C-25, followed by purification on high-performance gel filtration Shim-pack Diol-150 column. DbTx is a single-chain PLA2 toxin with approximate mol. wt 15,000 as determined by HPLC gel filtration and SDS-PAGE. It constitutes 12% of total venom-protein and is the main lethal component of Burmese Russell's viper venom with an LD50 i.p. (0.05 mg/kg) 12-fold greater than that of the whole venom (LD50 i.p. 0.6 mg/kg). DbTx produces neurotoxic symptoms in mice and exhibits potent oedema-inducing activity (minimum oedema dose 0.05 μg), indirect haemolytic activity and a strong myonecrotic activity, but no haemorrhagic activity. DbTx is cytotoxic to HeLa cells causing cytolysis of the cells 24 hr post-exposure to toxin (50 μg/ml). The first 20 N-terminal amino acid sequence (NFFQF AEMIV KMTGK EAVHS) shows a significant resemblance to those of the PLA2s from the venoms of Bulgarian viper (V. a. ammodytes) and Taiwan Russell's viper (V. r. formosensis).||Source Title:||Toxicon||URI:||http://scholarbank.nus.edu.sg/handle/10635/33805||ISSN:||00410101||DOI:||10.1016/0041-0101(94)00133-S|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Aug 21, 2019
WEB OF SCIENCETM
checked on Aug 14, 2019
checked on Aug 19, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.