Please use this identifier to cite or link to this item:
|Title:||Establishing clonal cell lines with endothelial-like potential from CD9hi, SSEA-1-cells in enbryonic stem cell-derived enbryoid bodies||Authors:||Lian, Q.
El, Oakley R.M.
|Issue Date:||2006||Citation:||Lian, Q., Que, J., Tan, E.K.W., Yu, F., Yeo, K.S., El, Oakley R.M., Lim, S.-K., Yin, Y., Salto-Tellez, M. (2006). Establishing clonal cell lines with endothelial-like potential from CD9hi, SSEA-1-cells in enbryonic stem cell-derived enbryoid bodies. PLoS ONE 1 (1) : -. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pone.0000006||Abstract:||Background. Differentiation of embryonic stem cells (ESCs) into specific cell types with minimal risk of teratoma formation could be efficiently directed by first reducing the differentiation potential of ESCs through the generation of clonal, self-renewing lineage-restricted stem cell lines. Efforts to isolate these stem cells are, however, mired in an impasse where the lack of purified lineage-restricted stem cells has hindered the identification of defining markers for these rare stem cells and, in turn, their isolation. Methodology/Principal Findings. We describe here a method for the isolation of clonal lineage-restricted cell lines with endothelial potential from ESCs through a combination of empirical and rational evidence-based methods. Using an empirical protocol that we have previously developed to generate embryo-derived RoSH lines with endothelial potential, we first generated E-RoSH lines from mouse ESC-derived embryoid bodies (EBs). Despite originating from different mouse strains, RoSH and E- RoSH lines have similar gene expression profiles (r2 = 0.93) while that between ERoSH and ESCs was 0.83. In silico gene expression analysis predicted that like RoSH cells, E-RoSH cells have an increased propensity to differentiate into vasculature. Unlike their parental ESCs, E-RoSH cells did not form teratomas and differentiate efficiently into endothelial-like cells in vivo and in vitro. Gene expression and FACS analysis revealed that RoSH and E-RoSH cells are CD9hi, SSEA-1 while ESCs are CD9lo, SSEA-1+. Isolation of CD9hi, SSEA-1 cells that constituted 1%-10% of EB-derived cultures generated an E-RoSH-like culture with an identical E-RoSH-like gene expression profile (r2 = 0.95) and a propensity to differentiate into endothelial-like cells. Conclusions. By combining empirical and rational evidence-based methods, we identified definitive selectable surface antigens for the isolation and propagation of lineage-restricted stem cells with endothelial-like potential from mouse ESCs. © 2006 Lian et al.||Source Title:||PLoS ONE||URI:||http://scholarbank.nus.edu.sg/handle/10635/33155||ISSN:||19326203||DOI:||10.1371/journal.pone.0000006|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
|10_1371_journal_pone_0000006.pdf||481.89 kB||Adobe PDF|
checked on Dec 3, 2019
WEB OF SCIENCETM
checked on Nov 25, 2019
checked on Dec 2, 2019
checked on Dec 2, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.