Please use this identifier to cite or link to this item: https://doi.org/10.1016/0378-4347(92)80445-V
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dc.titleHigh-performance liquid chromatographic method for routine determination of vitamins A and E and β-carotene in plasma
dc.contributor.authorLee, B.L.
dc.contributor.authorChua, S.C.
dc.contributor.authorOng, H.Y.
dc.contributor.authorOng, C.N.
dc.date.accessioned2012-04-02T06:51:48Z
dc.date.available2012-04-02T06:51:48Z
dc.date.issued1992
dc.identifier.citationLee, B.L., Chua, S.C., Ong, H.Y., Ong, C.N. (1992). High-performance liquid chromatographic method for routine determination of vitamins A and E and β-carotene in plasma. Journal of Chromatography - Biomedical Applications 581 (1) : 41-47. ScholarBank@NUS Repository. https://doi.org/10.1016/0378-4347(92)80445-V
dc.identifier.issn03784347
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/31715
dc.description.abstractA simple and reliable reversed-phase high-performance liquid chromatographic (HPLC) method for the routine determination of vitamins A and E and β-carotene in plasma (or serum) with wavelength-programmed ultraviolet-visible absorbance detection is described. A 200-μl aliquot of serum or plasma sample, after deproteinization with ethanol, and containing tocopherol acetate as internal standard, was extracted with butanol-ethyl acetate. Sodium sulphate was added for dehydration. Analytes of extracted samples were found to be stable for at least four days. A 10-μl aliquot of this organic extract was used for HPLC analysis. The mobile phase was methanol-butanol-water (89.5:5:5.5, v/v) and the flow-rate was set at 1.5 ml/min. The analytes of interest were well separated from other plasma constituents within 22 min at 45°C. The lowest detection limits of vitamins A and E and β-carotene were 0.02, 0.5 and 0.1 μg/ml, respectively. The recovery and reproducibility of the present method were around 90%. The method is sensitive, specific and can be used for epidemiological studies and for routine determination of vitamin deficiency. Several important factors that may affect the analysis are also discussed in this paper.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/0378-4347(92)80445-V
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCOMMUNITY,OCCUPATIONAL & FAMILY MEDICINE
dc.description.doi10.1016/0378-4347(92)80445-V
dc.description.sourcetitleJournal of Chromatography - Biomedical Applications
dc.description.volume581
dc.description.issue1
dc.description.page41-47
dc.description.codenJCBAD
dc.identifier.isiutA1992JR64500005
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