Please use this identifier to cite or link to this item: https://doi.org/10.1016/0956-5663(94)80122-3
DC FieldValue
dc.titleEffects of pre-conditioning and microbial composition on the sensing efficacy of a BOD biosensor
dc.contributor.authorLi, F.
dc.contributor.authorTan, C.
dc.contributor.authorLee, Y.K.
dc.date.accessioned2012-03-28T06:06:27Z
dc.date.available2012-03-28T06:06:27Z
dc.date.issued1994
dc.identifier.citationLi, F., Tan, C., Lee, Y.K. (1994). Effects of pre-conditioning and microbial composition on the sensing efficacy of a BOD biosensor. Biosensors and Bioelectronics 9 (3) : 197-205. ScholarBank@NUS Repository. https://doi.org/10.1016/0956-5663(94)80122-3
dc.identifier.issn09565663
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/31499
dc.description.abstractSensitivity and the linear range of BOD measurement of a microbial BOD sensor using a mixed cell population of Bacillus subtilis and Bacillus licheniformis 7B depend significantly on the proportion of the two microorganisms and on the cell population immobilized on the biofilm. Optimum characteristics were obtained with equal proportion of the two microorganisms and an immobilized cell population of not less than 108 in the biofilm. Preconditioning of newly installed biofilm was best carried out in high BOD solutions and the time required to fully activate the microbial system decreased with increasing cell population. Fully activated biofilm remained stable and gave reproducible results for over 200 measurements covering a time period of more than 2 months. The technique of cell immobilization used in the present study provided a close control of the population and composition of the two microorganisms in the biofilm. Consistent and reproducible quality biofilms can therefore be produced for the preparation of the microbial BOD sensor.
dc.description.abstractSensitivity and the linear range of BOD measurement of a microbial BOD sensor using a mixed cell population of Bacillus subtilis and Bacillus licheniformis 7B depend significantly on the proportion of the two microorganisms and on the cell population immobilized on the biofilm. Optimum characteristics were obtained with equal proportion of the two microorganisms and an immobilized cell population of not less than 108 on the biofilm. Pre-conditioning of newly installed biofilm was best carried out in high BOD solutions and the time required to fully activate the microbial system decreased with increasing cell population. Fully activated biofilm remained stable and gave reproducible results for over 200 measurements covering a time period of more than two months. The technique of cell immobilization used in the present study provided a close control of the population and composition of the two microorganisms in the biofilm. Consistent and reproducible quality biofilms can therefore be produced for the preparation of the microbial BOD sensor.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/0956-5663(94)80122-3
dc.publisherPubl by Elsevier Science Publ Ltd
dc.sourceScopus
dc.subjectBiofilm characterization
dc.subjectBod sensor
dc.subjectSensor pre-conditioning
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.contributor.departmentANATOMY
dc.description.doi10.1016/0956-5663(94)80122-3
dc.description.sourcetitleBiosensors and Bioelectronics
dc.description.volume9
dc.description.issue3
dc.description.page197-205
dc.description.codenBBIOE
dc.identifier.isiutA1994NP32300009
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