Please use this identifier to cite or link to this item: https://doi.org/10.1016/0378-1119(96)00133-3
DC FieldValue
dc.titleIS1394 from Pseudomonas alcaligenes N.C.I.B. 9867: Identification and characterization of a member of the IS30 family of insertion elements
dc.contributor.authorYeo, C.C.
dc.contributor.authorPoh, C.L.
dc.date.accessioned2012-03-28T05:46:31Z
dc.date.available2012-03-28T05:46:31Z
dc.date.issued1996
dc.identifier.citationYeo, C.C., Poh, C.L. (1996). IS1394 from Pseudomonas alcaligenes N.C.I.B. 9867: Identification and characterization of a member of the IS30 family of insertion elements. Gene 175 (1-2) : 109-113. ScholarBank@NUS Repository. https://doi.org/10.1016/0378-1119(96)00133-3
dc.identifier.issn03781119
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/31095
dc.description.abstractA new insertion sequence designated IS1394 was isolated from Pseudomonas alcaligenes NCIB 9867 (P25X) by entrapment in plasmid pUCD800 which carries the Bacillus subtilis sacB and sacR genes. The 1100-bp sequence contains 27-bp inverted repeats with 4 bp mismatch and has one long open reading frame, spanning 92.1% of the entire IS. The deduced 338 amino-acid sequence demonstrated homology (varying from 65% to 78% similarity and 36-67% identity) to transposases encoded by the IS30 family of IS elements. Comparison of four different IS-sacB junction sequences showed that IS1394 generated 3-bp direct repeats of target DNA upon insertion. IS1394 is present in at least 10 copies in the P25X genome but none was detected in its endogenous plasmid pRA2. Hybridization experiments revealed that the distribution of IS1394 is limited to closely related strains, being present in three copies in Pseudomonas putida NCIB 9869 (P35X) and two copies in Pseudomonas alcaligenes ATCC type strain (ATCC 14904).
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/0378-1119(96)00133-3
dc.sourceScopus
dc.subjectDirect repeat
dc.subjectEntrapment vector
dc.subjectInsertion sequence
dc.subjectInverted repeat
dc.subjectTransposase
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/0378-1119(96)00133-3
dc.description.sourcetitleGene
dc.description.volume175
dc.description.issue1-2
dc.description.page109-113
dc.description.codenGENED
dc.identifier.isiutA1996VR39800018
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

11
checked on Aug 19, 2019

WEB OF SCIENCETM
Citations

10
checked on Jul 1, 2019

Page view(s)

124
checked on Aug 19, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.