Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.ejheart.2006.04.008
DC Field | Value | |
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dc.title | Improved angiogenic response in pig heart following ischaemic injury using human skeletal myoblast simultaneously expressing VEGF165and angiopoietin-1 | |
dc.contributor.author | Ye, L. | |
dc.contributor.author | Sim, E.K.W. | |
dc.contributor.author | Haider, H.Kh. | |
dc.contributor.author | Jiang, S. | |
dc.contributor.author | Tan, R.S. | |
dc.contributor.author | Ge, R. | |
dc.contributor.author | Law, P.K. | |
dc.date.accessioned | 2012-01-30T09:45:40Z | |
dc.date.available | 2012-01-30T09:45:40Z | |
dc.date.issued | 2007 | |
dc.identifier.citation | Ye, L., Sim, E.K.W., Haider, H.Kh., Jiang, S., Tan, R.S., Ge, R., Law, P.K. (2007). Improved angiogenic response in pig heart following ischaemic injury using human skeletal myoblast simultaneously expressing VEGF165and angiopoietin-1. European Journal of Heart Failure 9 (1) : 15-22. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ejheart.2006.04.008 | |
dc.identifier.issn | 13889842 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/30195 | |
dc.description.abstract | Objective: To achieve angiogenic interaction between VEGF165 and angiopoietin-1 (Ang-1) using a novel adenoviral bicistronic vector (Ad-Bic) encoding the two factors and delivered ex vivo using sex-mismatched human skeletal myoblasts. Methods and results: A myocardial infarction model was developed in 29 female pigs; randomised into four groups: DMEM (group-1, n = 6); Adenovirus null (Ad-null) vector-myoblast (group-2, n = 5); Ad-Ang-1 myoblast (group 3, n = 7) and Ad-Bic-myoblast (group-4, n = 11). Three weeks later, 5 ml DMEM without myoblasts or containing 3 × 108 myoblasts carrying lac-z gene and transduced with Ad-null, Ad-Ang-1 or Ad-Bic were injected intra-myocardially in and around the infarct. 2D-echocardiography and fluorescent microsphere studies 6- and 12-weeks post-treatment revealed significantly improved cardiac performance and regional blood flow in groups 3 and 4. Histological studies and Y-chromosome analysis revealed extensive survival of lac-z positive myoblasts staining positive for human proteins in the pig heart. ELISA, immunostaining and RT-PCR revealed that Ad-Bic transduced myoblasts concomitantly but transiently expressed hVEGF165 and Ang-1 both in vitro and in vivo. Double fluorescent immunostaining of the tissue sections for vWFactor-III and smooth muscle actin showed significantly higher vascular density of mature blood vessels per low power microscopic field in groups 3 and 4 at 6- and 12-weeks. Conclusion: Our combined approach led to enhanced angiogenesis with a greater percentage of functionally mature blood vessels in a porcine heart. © 2006 European Society of Cardiology. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.ejheart.2006.04.008 | |
dc.source | Scopus | |
dc.subject | Angiogenesis | |
dc.subject | Gene therapy | |
dc.subject | Infarction | |
dc.subject | Skeletal myoblast | |
dc.type | Article | |
dc.contributor.department | BIOLOGICAL SCIENCES | |
dc.contributor.department | NATIONAL UNIVERSITY MEDICAL INSTITUTES | |
dc.contributor.department | SURGERY | |
dc.description.doi | 10.1016/j.ejheart.2006.04.008 | |
dc.description.sourcetitle | European Journal of Heart Failure | |
dc.description.volume | 9 | |
dc.description.issue | 1 | |
dc.description.page | 15-22 | |
dc.description.coden | EJHFF | |
dc.identifier.isiut | 000244422900005 | |
Appears in Collections: | Staff Publications |
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