Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.scr.2008.07.006
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dc.titleGenerating mESC-derived insulin-producing cell lines through an intermediate lineage-restricted progenitor line
dc.contributor.authorLi, G.
dc.contributor.authorLuo, R.
dc.contributor.authorZhang, J.
dc.contributor.authorLian, Q.
dc.contributor.authorXie, F.
dc.contributor.authorLim, S.K.
dc.contributor.authorSalto-Tellez, M.
dc.contributor.authorYeo, K.S.
dc.contributor.authorTan, E.K.W.
dc.contributor.authorCaille, D.
dc.contributor.authorMeda, P.
dc.contributor.authorKon, O.L.
dc.date.accessioned2012-01-30T09:44:58Z
dc.date.available2012-01-30T09:44:58Z
dc.date.issued2009
dc.identifier.citationLi, G., Luo, R., Zhang, J., Lian, Q., Xie, F., Lim, S.K., Salto-Tellez, M., Yeo, K.S., Tan, E.K.W., Caille, D., Meda, P., Kon, O.L. (2009). Generating mESC-derived insulin-producing cell lines through an intermediate lineage-restricted progenitor line. Stem Cell Research 2 (1) : 41-55. ScholarBank@NUS Repository. https://doi.org/10.1016/j.scr.2008.07.006
dc.identifier.issn18735061
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/30146
dc.description.abstractGenerating surrogate insulin-producing cells from embryonic stem cells (ESCs) through in vitro replication of successive steps during pancreatic development has been challenging . Here we describe a novel reproducible protocol to establish homogeneous and scalable insulin-producing cell lines from mouse (m) ESCs via differentiation of the previously described lineage-restricted clonal mESC-derived E-RoSH cells. Unlike their parental mESCs, E-RoSH cells expressed high levels of mesodermal and endodermal genes. Nutrient depletion in the presence of nicotinamide inhibited proliferation of E-RoSH cells and induced differentiation into heterogeneous cultures comprising vascular-like structures that produced detectable levels of insulin and C-peptide in an equimolar ratio. Limiting dilution of these cultures resulted in the isolation of eight independent insulin-producing cell lines in five experiments. All these lines were cloned and shown to be amenable to repeated cycles of freeze and thaw and to replicate for months with a doubling time of 3-4 days. Under such conditions, the cultured cells exhibited genomic, structural, biochemical, and pharmacological properties of pancreatic β cells, including storage of an equimolar ratio of insulin and C-peptide in granules and release of the contents of these organelles through a glucose-sensitive machinery. After transplantation, these cells reversed hyperglycemia in streptozotocin-treated SCID mice and did not form teratomas. © 2008.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.scr.2008.07.006
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentSURGERY
dc.contributor.departmentNATIONAL UNIVERSITY MEDICAL INSTITUTES
dc.description.doi10.1016/j.scr.2008.07.006
dc.description.sourcetitleStem Cell Research
dc.description.volume2
dc.description.issue1
dc.description.page41-55
dc.identifier.isiut000269909000006
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