Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.scr.2008.07.006
DC Field | Value | |
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dc.title | Generating mESC-derived insulin-producing cell lines through an intermediate lineage-restricted progenitor line | |
dc.contributor.author | Li, G. | |
dc.contributor.author | Luo, R. | |
dc.contributor.author | Zhang, J. | |
dc.contributor.author | Lian, Q. | |
dc.contributor.author | Xie, F. | |
dc.contributor.author | Lim, S.K. | |
dc.contributor.author | Salto-Tellez, M. | |
dc.contributor.author | Yeo, K.S. | |
dc.contributor.author | Tan, E.K.W. | |
dc.contributor.author | Caille, D. | |
dc.contributor.author | Meda, P. | |
dc.contributor.author | Kon, O.L. | |
dc.date.accessioned | 2012-01-30T09:44:58Z | |
dc.date.available | 2012-01-30T09:44:58Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Li, G., Luo, R., Zhang, J., Lian, Q., Xie, F., Lim, S.K., Salto-Tellez, M., Yeo, K.S., Tan, E.K.W., Caille, D., Meda, P., Kon, O.L. (2009). Generating mESC-derived insulin-producing cell lines through an intermediate lineage-restricted progenitor line. Stem Cell Research 2 (1) : 41-55. ScholarBank@NUS Repository. https://doi.org/10.1016/j.scr.2008.07.006 | |
dc.identifier.issn | 18735061 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/30146 | |
dc.description.abstract | Generating surrogate insulin-producing cells from embryonic stem cells (ESCs) through in vitro replication of successive steps during pancreatic development has been challenging . Here we describe a novel reproducible protocol to establish homogeneous and scalable insulin-producing cell lines from mouse (m) ESCs via differentiation of the previously described lineage-restricted clonal mESC-derived E-RoSH cells. Unlike their parental mESCs, E-RoSH cells expressed high levels of mesodermal and endodermal genes. Nutrient depletion in the presence of nicotinamide inhibited proliferation of E-RoSH cells and induced differentiation into heterogeneous cultures comprising vascular-like structures that produced detectable levels of insulin and C-peptide in an equimolar ratio. Limiting dilution of these cultures resulted in the isolation of eight independent insulin-producing cell lines in five experiments. All these lines were cloned and shown to be amenable to repeated cycles of freeze and thaw and to replicate for months with a doubling time of 3-4 days. Under such conditions, the cultured cells exhibited genomic, structural, biochemical, and pharmacological properties of pancreatic β cells, including storage of an equimolar ratio of insulin and C-peptide in granules and release of the contents of these organelles through a glucose-sensitive machinery. After transplantation, these cells reversed hyperglycemia in streptozotocin-treated SCID mice and did not form teratomas. © 2008. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.scr.2008.07.006 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | SURGERY | |
dc.contributor.department | NATIONAL UNIVERSITY MEDICAL INSTITUTES | |
dc.description.doi | 10.1016/j.scr.2008.07.006 | |
dc.description.sourcetitle | Stem Cell Research | |
dc.description.volume | 2 | |
dc.description.issue | 1 | |
dc.description.page | 41-55 | |
dc.identifier.isiut | 000269909000006 | |
Appears in Collections: | Staff Publications |
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