Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0142-9612(01)00191-0
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dc.titleMulti-layered microcapsules for cell encapsulation
dc.contributor.authorChia, S.M.
dc.contributor.authorNg, M.L.
dc.contributor.authorYu, H.
dc.contributor.authorWan, A.C.A.
dc.contributor.authorQuek, C.H.
dc.contributor.authorXu, X.
dc.contributor.authorShen, L.
dc.contributor.authorLeong, K.W.
dc.contributor.authorMao, H.Q.
dc.date.accessioned2011-12-07T08:16:28Z
dc.date.available2011-12-07T08:16:28Z
dc.date.issued2002
dc.identifier.citationChia, S.M., Ng, M.L., Yu, H., Wan, A.C.A., Quek, C.H., Xu, X., Shen, L., Leong, K.W., Mao, H.Q. (2002). Multi-layered microcapsules for cell encapsulation. Biomaterials 23 (3) : 849-856. ScholarBank@NUS Repository. https://doi.org/10.1016/S0142-9612(01)00191-0
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/29692
dc.description.abstractMechanical stability, complete encapsulation, selective permeability, and suitable extra-cellular microenvironment, are the major considerations in designing microcapsules for cell encapsulation. We have developed four types of multi-layered microcapsules that allow selective optimization of these parameters. Primary hepatocytes were used as model cells to test these different microcapsule configurations. Type-I microcapsules with an average diameter of 400 μm were formed by complexing modified collagen with a ter-polymer shell of 2-hydroxyethyl methylacrylate (HEMA), methacrylic acid (MAA) and methyl methacrylate (MMA), resulting in a capsule thickness of 2-5 μm. Cells in these microcapsules exhibited improved cellular functions over those cultured on collagen monolayers. Type-II microcapsules were formed by encapsulating the Type-I microcapsules in another 2-5 μm ter-polymer shell and a ∼5 μm collagen layer between the two ter-polymer shells to ensure complete cell encapsulation. Type-III microcapsules comprised of a macro-porous exoskeleton with materials such as alumina sol-gel coated on the Type-I microcapsules. Nano-indendation assay indicated an improved mechanical stability over the Type-I microcapsules. Type-IV microcapsules were created by encapsulating Type-III microcapsules in another 2-5 μm ter-polymer shell, with the aim of imparting a negatively charged smooth surface to minimize plasma protein absorption and ensure complete cell encapsulation. The permeability for nutrient exchange, cellular functions in terms of urea production and mechanical stability of the microcapsules were characterized. The advantages and limitations of these microcapsules for tissue engineering are discussed. © 2001 Elsevier Science Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0142-9612(01)00191-0
dc.sourceScopus
dc.subjectCell microencapsulation
dc.subjectCeramic sol-gel
dc.subjectExoskeleton
dc.subjectNano-indentation
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.contributor.departmentPHYSIOLOGY
dc.contributor.departmentMATERIALS SCIENCE
dc.description.doi10.1016/S0142-9612(01)00191-0
dc.description.sourcetitleBiomaterials
dc.description.volume23
dc.description.issue3
dc.description.page849-856
dc.description.codenBIMAD
dc.identifier.isiut000172640000026
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