Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.jneumeth.2006.05.017
DC Field | Value | |
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dc.title | pH dependent high transfection efficiency of mouse neuroblastomas using TransFectin | |
dc.contributor.author | Chong, K.W.Y. | |
dc.contributor.author | Seet, S.J. | |
dc.contributor.author | Cheung, N.S. | |
dc.contributor.author | Lee, A.Y.-W. | |
dc.contributor.author | Koay, E.S.C. | |
dc.date.accessioned | 2011-11-29T06:10:41Z | |
dc.date.available | 2011-11-29T06:10:41Z | |
dc.date.issued | 2006 | |
dc.identifier.citation | Chong, K.W.Y., Seet, S.J., Cheung, N.S., Lee, A.Y.-W., Koay, E.S.C. (2006). pH dependent high transfection efficiency of mouse neuroblastomas using TransFectin. Journal of Neuroscience Methods 158 (1) : 56-63. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jneumeth.2006.05.017 | |
dc.identifier.issn | 01650270 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/28892 | |
dc.description.abstract | Mouse neuroblastoma cell lines are often used in lieu of mouse primary neurons in ex vivo experiments, as they provide an easier platform for transfection, compared to the latter. A well-known inherent problem with this strategy is the relatively low transfection efficiency (15-30%) of mouse neuroblastoma cell lines such as neuro-2A and N1E-115. We were able to improve the transfection efficiency of these cell lines by using the cationic lipid reagent, TransFectin™ (Bio-Rad, Hercules, CA, USA) to optimise the transfection conditions. Our results, based on fluorescence intensity determinations and Western blotting for enhanced green fluorescence protein (EGFP) over-expression in neuro-2A, demonstrated that pH is a crucial factor in determining the transfection efficiency. Under pH-optimised transfection conditions, flow cytometric analysis revealed high EGFP transfection efficiencies of 76.4 ± 0.5 and 60.9 ± 0.6% for neuro-2A and N1E-115, respectively. Notably, the optimised TransFectin™-based transfection system did not result in any detectable cytotoxicity to the mouse neuroblastomas. The resultant optimised system is economical, easy to use and does not require any specialised equipment. © 2006 Elsevier B.V. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jneumeth.2006.05.017 | |
dc.source | Scopus | |
dc.subject | Mouse | |
dc.subject | N1E-115 | |
dc.subject | Neuro-2A | |
dc.subject | Neuroblastoma | |
dc.subject | Optimised procedure | |
dc.subject | pH | |
dc.subject | TransFectin™ | |
dc.subject | Transfection | |
dc.type | Article | |
dc.contributor.department | BIOCHEMISTRY | |
dc.contributor.department | PHYSIOLOGY | |
dc.contributor.department | PATHOLOGY | |
dc.description.doi | 10.1016/j.jneumeth.2006.05.017 | |
dc.description.sourcetitle | Journal of Neuroscience Methods | |
dc.description.volume | 158 | |
dc.description.issue | 1 | |
dc.description.page | 56-63 | |
dc.description.coden | JNMED | |
dc.identifier.isiut | 000241931600008 | |
Appears in Collections: | Staff Publications |
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