Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.bbrc.2006.02.154
DC Field | Value | |
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dc.title | Evidence for the formation of a novel nitrosothiol from the gaseous mediators nitric oxide and hydrogen sulphide | |
dc.contributor.author | Whiteman, M. | |
dc.contributor.author | Chu, S.H. | |
dc.contributor.author | Siau, J.L. | |
dc.contributor.author | Li, L. | |
dc.contributor.author | Bhatia, M. | |
dc.contributor.author | Moore, P.K. | |
dc.contributor.author | Kostetski, I. | |
dc.date.accessioned | 2011-11-29T06:10:01Z | |
dc.date.available | 2011-11-29T06:10:01Z | |
dc.date.issued | 2006 | |
dc.identifier.citation | Whiteman, M., Chu, S.H., Siau, J.L., Li, L., Bhatia, M., Moore, P.K., Kostetski, I. (2006). Evidence for the formation of a novel nitrosothiol from the gaseous mediators nitric oxide and hydrogen sulphide. Biochemical and Biophysical Research Communications 343 (1) : 303-310. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2006.02.154 | |
dc.identifier.issn | 0006291X | |
dc.identifier.issn | 10902104 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/28833 | |
dc.description.abstract | The gaseous mediators hydrogen sulphide (H2S) and nitric oxide (.NO) are synthesised in the body from l-cysteine and l-arginine, respectively. In the cardiovascular system, .NO is an important regulator of vascular tone and its over- or under-production has been linked to a variety of diseases. The physiological significance of H2S is not yet clear but, like .NO, it exhibits vasodilator activity and may play a part in septic and haemorrhagic shock, hypertension, regulation of cardiac contractility, and in inflammation. To date, there have been no reports of a chemical interaction between H2S and .NO. Here we show that incubation of the H2S donor, sodium hydrosulphide, with a range of .NO donors and .NO gas in vitro leads to the formation of a nitrosothiol molecule as determined by a combination of techniques; electron paramagnetic resonance, amperometry, and measurement of nitrite. We further show that this nitrosothiol did not induce cGMP accumulation in cultured RAW264.7 cells unless .NO was released with Cu 2+. Finally, using liver homogenates from LPS treated rats we present evidence for the endogenous formation of this nitrosothiol. These findings provide the first evidence for the formation of a novel nitrosothiol generated by reaction between H2S and .NO. We propose that generation of this nitrosothiol in the body may regulate the physiological effects of both .NO and H2S. © 2006 Elsevier Inc. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2006.02.154 | |
dc.source | Scopus | |
dc.subject | Cystathionine-γ-synthase | |
dc.subject | Cysthionine-β-synthase | |
dc.subject | Hydrogen sulphide | |
dc.subject | Nitric oxide | |
dc.subject | Peroxynitrite | |
dc.subject | Reactive oxygen species | |
dc.type | Article | |
dc.contributor.department | BIOCHEMISTRY | |
dc.contributor.department | DIVISION OF BIOENGINEERING | |
dc.contributor.department | PHARMACOLOGY | |
dc.description.doi | 10.1016/j.bbrc.2006.02.154 | |
dc.description.sourcetitle | Biochemical and Biophysical Research Communications | |
dc.description.volume | 343 | |
dc.description.issue | 1 | |
dc.description.page | 303-310 | |
dc.description.coden | BBRCA | |
dc.identifier.isiut | 000236659300042 | |
Appears in Collections: | Staff Publications |
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