Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bbrc.2006.02.154
DC FieldValue
dc.titleEvidence for the formation of a novel nitrosothiol from the gaseous mediators nitric oxide and hydrogen sulphide
dc.contributor.authorWhiteman, M.
dc.contributor.authorChu, S.H.
dc.contributor.authorSiau, J.L.
dc.contributor.authorLi, L.
dc.contributor.authorBhatia, M.
dc.contributor.authorMoore, P.K.
dc.contributor.authorKostetski, I.
dc.date.accessioned2011-11-29T06:10:01Z
dc.date.available2011-11-29T06:10:01Z
dc.date.issued2006
dc.identifier.citationWhiteman, M., Chu, S.H., Siau, J.L., Li, L., Bhatia, M., Moore, P.K., Kostetski, I. (2006). Evidence for the formation of a novel nitrosothiol from the gaseous mediators nitric oxide and hydrogen sulphide. Biochemical and Biophysical Research Communications 343 (1) : 303-310. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2006.02.154
dc.identifier.issn0006291X
dc.identifier.issn10902104
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/28833
dc.description.abstractThe gaseous mediators hydrogen sulphide (H2S) and nitric oxide (.NO) are synthesised in the body from l-cysteine and l-arginine, respectively. In the cardiovascular system, .NO is an important regulator of vascular tone and its over- or under-production has been linked to a variety of diseases. The physiological significance of H2S is not yet clear but, like .NO, it exhibits vasodilator activity and may play a part in septic and haemorrhagic shock, hypertension, regulation of cardiac contractility, and in inflammation. To date, there have been no reports of a chemical interaction between H2S and .NO. Here we show that incubation of the H2S donor, sodium hydrosulphide, with a range of .NO donors and .NO gas in vitro leads to the formation of a nitrosothiol molecule as determined by a combination of techniques; electron paramagnetic resonance, amperometry, and measurement of nitrite. We further show that this nitrosothiol did not induce cGMP accumulation in cultured RAW264.7 cells unless .NO was released with Cu 2+. Finally, using liver homogenates from LPS treated rats we present evidence for the endogenous formation of this nitrosothiol. These findings provide the first evidence for the formation of a novel nitrosothiol generated by reaction between H2S and .NO. We propose that generation of this nitrosothiol in the body may regulate the physiological effects of both .NO and H2S. © 2006 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2006.02.154
dc.sourceScopus
dc.subjectCystathionine-γ-synthase
dc.subjectCysthionine-β-synthase
dc.subjectHydrogen sulphide
dc.subjectNitric oxide
dc.subjectPeroxynitrite
dc.subjectReactive oxygen species
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.contributor.departmentDIVISION OF BIOENGINEERING
dc.contributor.departmentPHARMACOLOGY
dc.description.doi10.1016/j.bbrc.2006.02.154
dc.description.sourcetitleBiochemical and Biophysical Research Communications
dc.description.volume343
dc.description.issue1
dc.description.page303-310
dc.description.codenBBRCA
dc.identifier.isiut000236659300042
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