Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.nimb.2006.03.073
DC FieldValue
dc.titleA nuclear microscopy study of trace elements Ca, Fe, Zn and Cu in atherosclerosis
dc.contributor.authorWatt, F.
dc.contributor.authorRajendran, R.
dc.contributor.authorRen, M.Q.
dc.contributor.authorHalliwell, B.
dc.contributor.authorTan, B.K.H.
dc.date.accessioned2011-11-29T06:09:30Z
dc.date.available2011-11-29T06:09:30Z
dc.date.issued2006
dc.identifier.citationWatt, F., Rajendran, R., Ren, M.Q., Halliwell, B., Tan, B.K.H. (2006). A nuclear microscopy study of trace elements Ca, Fe, Zn and Cu in atherosclerosis. Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms 249 (1-2 SPEC. ISS.) : 646-652. ScholarBank@NUS Repository. https://doi.org/10.1016/j.nimb.2006.03.073
dc.identifier.issn0168583X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/28791
dc.description.abstractQuantitative mapping of trace elements Ca, Fe, Zn and Cu can be achieved in biological tissue using a nuclear microprobe. Presented here is a brief review of the work we have carried out in the last decade using the nuclear microscope to try and elucidate the role of trace elements Fe, Zn, Cu and Ca in induced atherosclerosis in New Zealand White rabbits fed on a 1% cholesterol diet. The lesions were studied using nuclear microscopy, incorporating a combination of ion beam techniques: particle induced X-ray emission (PIXE), Rutherford backscattering spectrometry (RBS) and scanning transmission ion microscopy (STIM). Iron is present in early lesions at concentrations around seven times higher than the artery wall. Measurements of localized lesion iron concentrations were observed to be highly correlated with the depth of the lesion in the artery wall for each individual animal, implying that local elevated concentrations may provide an accelerated process of atherosclerosis in specific regions of the artery. When the rabbits were kept mildly anaemic, thereby reducing iron levels in the lesion, the progression of the disease was significantly slowed. Iron chelation using desferal showed that early treatment (three weeks into the high fat diet) for relatively long periods (nine weeks) significantly retarded the progression of the disease. Zinc is depleted in the lesion and is also observed to be anti-correlated with local lesion development and feeding the rabbits on a high fat diet with zinc supplements inhibited lesion development, although since no significant increase in lesion zinc levels was measured, this anti-atherosclerotic effect may be indirect. Copper, measured at low levels (∼3 ppm) in the early lesion, is also depleted compared to the artery wall, suggesting that it is not a major factor in atherogenesis. Calcium is also depleted in early lesions, although at a later stage mineral deposition (hydroxyapatite) is observed to take place in the lesion/artery wall interface (intima), and subsequently in the lesion. These results are consistent with the hypotheses that iron plays a role in atherosclerosis probably through the production of free radicals and that zinc has an indirect protective effect. Copper appears to have a minor role due to its low lesion concentrations and hydroxyapatite deposition is a relatively late event. © 2006.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.nimb.2006.03.073
dc.sourceScopus
dc.subjectAtherosclerosis
dc.subjectCalcium
dc.subjectCopper
dc.subjectElemental mapping
dc.subjectIron
dc.subjectNuclear microprobe
dc.subjectNuclear microscopy
dc.subjectZinc
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.contributor.departmentPHYSICS
dc.contributor.departmentPHARMACOLOGY
dc.description.doi10.1016/j.nimb.2006.03.073
dc.description.sourcetitleNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
dc.description.volume249
dc.description.issue1-2 SPEC. ISS.
dc.description.page646-652
dc.description.codenNIMBE
dc.identifier.isiut000239545000158
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

32
checked on Jan 17, 2020

WEB OF SCIENCETM
Citations

36
checked on Jan 10, 2020

Page view(s)

254
checked on Dec 31, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.