Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.bbrc.2008.02.116
DC Field | Value | |
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dc.title | Nephrotoxic cell death by diclofenac and meloxicam | |
dc.contributor.author | Ng, L.E. | |
dc.contributor.author | Halliwell, B. | |
dc.contributor.author | Wong, K.P. | |
dc.date.accessioned | 2011-11-29T05:57:27Z | |
dc.date.available | 2011-11-29T05:57:27Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Ng, L.E., Halliwell, B., Wong, K.P. (2008). Nephrotoxic cell death by diclofenac and meloxicam. Biochemical and Biophysical Research Communications 369 (3) : 873-877. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2008.02.116 | |
dc.identifier.issn | 0006291X | |
dc.identifier.issn | 10902104 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/28621 | |
dc.description.abstract | The nephrotoxicity of diclofenac, a non-steroidal anti-inflammatory drug that inhibits both isoforms of cyclooxygenase (COX) has been reported to be fatal to vultures but this was not so with meloxicam which is COX-2 selective. Our study showed that diclofenac was more toxic than meloxicam to both the proximal tubular LLC-PK1 cells and the distal tubular Madin-Darby canine kidney type II (MDCKII) cells, and that LLC-PK1 cells were more susceptible. Exposure of MDCKII cells to meloxicam caused activation of caspase-9/-3 and release of cytochrome c. These observations together with a positive annexin V-FITC staining implicate an intrinsic mitochondrial cell death pathway by apoptosis. Diclofenac-treated MDCKII cells on the other hand showed extensive propidium iodide staining, suggestive of cell death by necrosis. The mode of cell death in LLC-PK1 cells was however less well-defined with positive annexin V-FITC staining but minimal increase in caspase-3 activity alluding to a caspase-independent pathway. © 2008 Elsevier Inc. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bbrc.2008.02.116 | |
dc.source | Scopus | |
dc.subject | Apoptosis | |
dc.subject | Caspase | |
dc.subject | Cytochrome c | |
dc.subject | Diclofenac | |
dc.subject | LLC-PK1 | |
dc.subject | MDCKII | |
dc.subject | Meloxicam | |
dc.type | Article | |
dc.contributor.department | BIOCHEMISTRY | |
dc.description.doi | 10.1016/j.bbrc.2008.02.116 | |
dc.description.sourcetitle | Biochemical and Biophysical Research Communications | |
dc.description.volume | 369 | |
dc.description.issue | 3 | |
dc.description.page | 873-877 | |
dc.description.coden | BBRCA | |
dc.identifier.isiut | 000254846500016 | |
Appears in Collections: | Staff Publications |
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