Please use this identifier to cite or link to this item:
|Title:||Measurement of F2-isoprostanes, hydroxyeicosatetraenoic products, and oxysterols from a single plasma sample||Authors:||Lee, C.-Y.J.
|Issue Date:||2008||Citation:||Lee, C.-Y.J., Huang, S.H., Jenner, A.M., Halliwell, B. (2008). Measurement of F2-isoprostanes, hydroxyeicosatetraenoic products, and oxysterols from a single plasma sample. Free Radical Biology and Medicine 44 (7) : 1314-1322. ScholarBank@NUS Repository. https://doi.org/10.1016/j.freeradbiomed.2007.12.026||Abstract:||Oxidized lipids such as F2-isoprostanes (F2-IsoPs), hydroxyeicosatetraenoic acid products (HETEs), and cholesterol oxidation products (COPs) are widely believed to be involved in multiple diseases. Usually, each product is measured individually in separate blood samples. In this study we describe a method allowing us to measure F2-IsoPs, HETEs, COPs, and arachidonate using a single sample. Plasma (1 ml) samples from healthy volunteers were diluted with heavy isotopic standards, hydrolyzed in alkali with organic solvent, and then subjected to anionic-exchange solid-phase extraction (SPE). After the SPE column was washed, hexane and hexane/ethyl acetate portions were collected and combined for COPs measurement. Thereafter the column was loaded with hexane/ethanol/acetic acid and fractions were collected for total F2-IsoPs, total HETEs, and arachidonate measurement. All compounds in the eluates were measured by gas chromatography-mass spectrometry. The efficiency of SPE and reproducibility for all compounds measured were high. Levels of total F2-IsoPs (0.45 ± 0.26 ng/ml (n = 157)), total HETEs (34.06 ± 16.35 ng/ml (n = 21)), total arachidonate (68.36 ± 24.45 μg/ml (n = 33)), and COPs (7-ketocholesterol, 12.25 ± 6.56 ng / ml; 7β-hydroxycholesterol, 6.32 ± 3.46 ng / ml; 7α-hydroxycholesterol, 15.06 ± 7.06 ng/ml; 24-hydroxycholesterol, 41.39 ± 18.22 ng/ml; and 27-hydroxycholesterol, 29.08 ± 16.79 ng/ml (n = 26)) were recorded in healthy subjects (age range 20 to 66 years; average male to female ratio 1:1). © 2007 Elsevier Inc. All rights reserved.||Source Title:||Free Radical Biology and Medicine||URI:||http://scholarbank.nus.edu.sg/handle/10635/28610||ISSN:||08915849||DOI:||10.1016/j.freeradbiomed.2007.12.026|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 18, 2019
WEB OF SCIENCETM
checked on Jul 11, 2019
checked on May 24, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.