Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.cardiores.2007.05.026
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dc.titleThe novel proangiogenic effect of hydrogen sulfide is dependent on Akt phosphorylation
dc.contributor.authorCai, W.-J.
dc.contributor.authorWang, M.-J.
dc.contributor.authorJin, H.-M.
dc.contributor.authorYao, T.
dc.contributor.authorZhu, Y.-C.
dc.contributor.authorMoore, P.K.
dc.date.accessioned2011-09-29T05:54:51Z
dc.date.available2011-09-29T05:54:51Z
dc.date.issued2007
dc.identifier.citationCai, W.-J., Wang, M.-J., Jin, H.-M., Yao, T., Zhu, Y.-C., Moore, P.K. (2007). The novel proangiogenic effect of hydrogen sulfide is dependent on Akt phosphorylation. Cardiovascular Research 76 (1) : 29-40. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cardiores.2007.05.026
dc.identifier.issn00086363
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/27300
dc.description.abstractObjective: Hydrogen sulfide (H2S) has been reported to be a gasotransmitter which regulates cardiovascular homeostasis. The present study aims to examine the hypothesis that hydrogen sulfide is able to promote angiogenesis. Methods: Angiogenesis was assessed using in vitro parameters (i.e. endothelial cell proliferation, adhesion, transwell migration assay, scratched wound healing and formation of tube-like structure) and in vivo by assessing neovascularization in mice. Phosphorylation of Akt was measured using Western blot analysis. Results: Exogenously administered NaHS (H2S donor) concentration-dependently (10-20 μmol/l) increased cell growth, migration, scratched wound healing and tube-like structure formation in cultured endothelial cells. These effects of NaHS on endothelial wound healing and tube-like structure formation were prevented by either the phosphatidylinositol 3-kinase (PI3K) inhibitor LY 294002 (5 μmol/l) or transfection of a dominant-negative mutant of Akt. NaHS increased Akt phosphorylation and this effect was also blocked by either LY 294002 or wortmannin (25 nmol/l). NaHS did not significantly alter the levels of vascular endothelial growth factor, mRNA expression of fibroblast growth factor and angiopoietin-1, or nitric oxide metabolites. NaHS treatment (10 and 50 μmol kg- 1 day- 1) significantly promoted neovascularization in vivo in mice. Conclusion: The present study reports a novel proangiogenic role of H2S which is dependent on activation of Akt. © 2007 European Society of Cardiology.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.cardiores.2007.05.026
dc.sourceScopus
dc.subjectAngiogenesis
dc.subjectEndothelial cells
dc.subjectMigration
dc.typeArticle
dc.contributor.departmentPHARMACOLOGY
dc.description.doi10.1016/j.cardiores.2007.05.026
dc.description.sourcetitleCardiovascular Research
dc.description.volume76
dc.description.issue1
dc.description.page29-40
dc.identifier.isiut000250025700006
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