Please use this identifier to cite or link to this item:
|Title:||Effect of hydrogen sulfide on intracellular calcium homeostasis in neuronal cells||Authors:||Yong, Q.C.
|Keywords:||L-type calcium channel
T-type calcium channel
|Issue Date:||2010||Citation:||Yong, Q.C., Choo, C.H., Tan, B.H., Low, C.-M., Bian, J.-S. (2010). Effect of hydrogen sulfide on intracellular calcium homeostasis in neuronal cells. Neurochemistry International 56 (3) : 508-515. ScholarBank@NUS Repository. https://doi.org/10.1016/j.neuint.2009.12.011||Abstract:||Hydrogen sulfide (H2S) is now known as a new biological mediator. In the present study, the effects of H2S on intracellular calcium ([Ca2+]i) in neuronal SH-SY5Y cells was investigated. In SH-SY5Y neuronal cells, NaHS, a H2S donor, concentration-dependently increased [Ca2+]i. The H2S-induced Ca2+ elevation was significantly attenuated by EGTA-treated calcium-free Krebs' solution. This elevation was also reduced by antagonists of L-type (verapamil and nifedipine), T-type (mibefradil) calcium channels and N-methyl-d-aspartate receptor (MK-801, AP-5 and ifenprodil). A 90% reduction in H2S-induced [Ca2+]i elevation was found in cells pretreated with combination of all three kinds of inhibitors. Depletion of intracellular Ca2+ store with thapsigargin or cyclopiazonic acid or blockade of ryanodine receptor with ruthenium red significantly attenuated the effect of H2S on [Ca2+]i. Inhibition of protein kinase A (PKA), phospholipase C (PLC) and protein kinase C (PKC) suppressed the H2S-elevated [Ca2+]i, suggesting that H2S may regulate [Ca2+]i via both PKA and PLC/PKC pathways. In conclusion, it was found in this study that H2S increased [Ca2+]i in SH-SY5Y neuronal cells by increasing Ca2+ influx via plasma membrane and in turn releasing calcium from intracellular calcium store. The findings in the present study provide the direct evidence that H2S may serve as a neuromodulator. © 2009 Elsevier Ltd.||Source Title:||Neurochemistry International||URI:||http://scholarbank.nus.edu.sg/handle/10635/27214||ISSN:||01970186||DOI:||10.1016/j.neuint.2009.12.011|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 19, 2019
WEB OF SCIENCETM
checked on Jul 19, 2019
checked on Jul 22, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.