Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.tiv.2007.05.003
DC Field | Value | |
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dc.title | Molecular and functional characterization of drug-metabolizing enzymes and transporter expression in the novel spontaneously immortalized human hepatocyte line HC-04 | |
dc.contributor.author | Lim, P.L.K. | |
dc.contributor.author | Latchoumycandane, C. | |
dc.contributor.author | Khoo, Y.M. | |
dc.contributor.author | Lee, H.S. | |
dc.contributor.author | Boelsterli, U.A. | |
dc.contributor.author | Tan, W. | |
dc.contributor.author | Tan, T.M.C. | |
dc.contributor.author | Mok, W.C. | |
dc.contributor.author | Lim, S.G. | |
dc.contributor.author | Sattabongkot, J. | |
dc.date.accessioned | 2011-09-27T05:45:01Z | |
dc.date.available | 2011-09-27T05:45:01Z | |
dc.date.issued | 2007 | |
dc.identifier.citation | Lim, P.L.K., Latchoumycandane, C., Khoo, Y.M., Lee, H.S., Boelsterli, U.A., Tan, W., Tan, T.M.C., Mok, W.C., Lim, S.G., Sattabongkot, J. (2007). Molecular and functional characterization of drug-metabolizing enzymes and transporter expression in the novel spontaneously immortalized human hepatocyte line HC-04. Toxicology in Vitro 21 (8) : 1390-1401. ScholarBank@NUS Repository. https://doi.org/10.1016/j.tiv.2007.05.003 | |
dc.identifier.issn | 08872333 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/27128 | |
dc.description.abstract | In toxicological research, immortalized human hepatocytes provide a useful alternative to primary hepatocytes because interindividual variability in the expression of drug-metabolizing enzymes and drug transporters can largely be eliminated. However, it is essential that the cell line retain the original phenotype. The purpose of this study was to characterize a novel spontaneously immortalized human hepatocyte cell line, HC-04, with respect to the transcript and functional protein expression profile for the major drug-metabolizing enzymes and transmembrane transporters. HC-04 cells retained hepatocyte-specific function including albumin production and ornithine transcarbamoylase and glucose-6-phosphatase activity. Most of the major CYP forms were expressed at basal levels and responsive to inducing agents. In particular, CYP3A4 was expressed abundantly, and HC-04 cells were able to metabolize the CYP3A4 probe, midazolam, at a rate similar to primary human hepatocytes. Furthermore, the major human sulfotransferase and UDP-glucuronosyltransferase forms, as well as members of the ABC and SLC transporter superfamilies, nuclear receptors, and hepatic transcription factors were also expressed. HC-04 cells readily responded to standard hepatotoxicants that are dependent on CYP-mediated bioactivation, while another, tumor-derived cell line remained refractory to the drug challenge. Collectively, HC-04 cells provide a reliable, stable, and reproducible model for biomechanistic studies in drug toxicology. © 2007 Elsevier Ltd. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.tiv.2007.05.003 | |
dc.source | Scopus | |
dc.subject | Drug toxicity in vitro | |
dc.subject | Drug transporters | |
dc.subject | Drug-metabolizing enzymes | |
dc.subject | Immortalized human hepatocytes | |
dc.type | Article | |
dc.contributor.department | BIOCHEMISTRY | |
dc.contributor.department | MEDICINE | |
dc.contributor.department | PHARMACOLOGY | |
dc.description.doi | 10.1016/j.tiv.2007.05.003 | |
dc.description.sourcetitle | Toxicology in Vitro | |
dc.description.volume | 21 | |
dc.description.issue | 8 | |
dc.description.page | 1390-1401 | |
dc.identifier.isiut | 000251558100005 | |
Appears in Collections: | Staff Publications |
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