Please use this identifier to cite or link to this item:
|Title:||Reduced transcriptional activity in individuals with IL-18 gene variants detected from functional but not association study||Authors:||Liang, X.H.
Reporter gene assay
|Issue Date:||2005||Citation:||Liang, X.H., Wang, D.Y., Cheung, W., Heng, C.K. (2005). Reduced transcriptional activity in individuals with IL-18 gene variants detected from functional but not association study. Biochemical and Biophysical Research Communications 338 (2) : 736-741. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2005.10.012||Abstract:||Genetic polymorphisms of IL-18 and its receptor were reported to be associated with elevated serum IgE levels, atopy, and/or asthma. However, conflicting results were observed in various association studies and functional activity of these polymorphisms remains unclear. A total of 393 unrelated subjects were involved in this study. Direct PCR-sequencing method was used to screen novel polymorphisms. The functional significance of these polymorphisms was investigated using reporter gene assay. Three known (-137, +113, and +127) polymorphisms in the IL-18 promoter were identified with a perfect linkage disequilibrium (Δ = 1, p < 0.001) among them. No significant difference in the genotype frequencies of these polymorphisms between atopy and atopic phenotypes in Singaporean Chinese, Malays, and Indians was observed. However, transcriptional activities were significantly increased in HepG2 cultured cells with wild-type IL-18 genotype (-137/G, +113/T, and +127/C) than mutated genotype (-137/C, +113/G, and +127/T). Although these polymorphisms appear to have no association with atopic phenotypes in our population, subsequent functional studies suggest that polymorphisms in the IL-18 promoter region could affect significantly its activity. © 2005 Elsevier Inc. All rights reserved.||Source Title:||Biochemical and Biophysical Research Communications||URI:||http://scholarbank.nus.edu.sg/handle/10635/26315||ISSN:||0006291X
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Feb 19, 2020
WEB OF SCIENCETM
checked on Feb 12, 2020
checked on Feb 19, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.