Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.freeradbiomed.2007.11.023
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dc.titleEffects of epigallocatechin-3-gallate on mitochondrial integrity and antioxidative enzyme activity in the aging process of human fibroblast
dc.contributor.authorMeng, Q.
dc.contributor.authorVelalar, C.N.
dc.contributor.authorRuan, R.
dc.date.accessioned2011-09-19T07:39:27Z
dc.date.available2011-09-19T07:39:27Z
dc.date.issued2008
dc.identifier.citationMeng, Q., Velalar, C.N., Ruan, R. (2008). Effects of epigallocatechin-3-gallate on mitochondrial integrity and antioxidative enzyme activity in the aging process of human fibroblast. Free Radical Biology and Medicine 44 (6) : 1032-1041. ScholarBank@NUS Repository. https://doi.org/10.1016/j.freeradbiomed.2007.11.023
dc.identifier.issn08915849
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/26286
dc.description.abstractMitochondrial integrity and antioxidative enzyme activity are two of the determinants of intracellular reactive oxygen species (ROS) accumulation probably underlying the aging mechanism. In this study, epigallocatechin-3-gallate (EGCG) was examined for its antiaging effect on human diploid fibroblasts (HDF). EGCG was evaluated for its cytotoxicity, and LC50 values were 78.0 and 84.4 μM for young and old HDF, respectively. HDF treated with EGCG at 25 and 50 μM for 24 h considerably increased catalase, superoxide dismutase (SOD)1, SOD2, and glutathione peroxidase gene expressions and their enzyme activities, thus protecting HDF against H2O2-induced oxidative damage, accompanied with decreased intracellular ROS accumulation and well-maintained mitochondrial potential. Moreover, HDF treated with EGCG at 12.5 μM for long term showed less intracellular ROS with higher mitochondrial potential, more intact mitochondrial DNA, much elevated antioxidative enzyme efficiency, and more juvenile cell status compared to those of the untreated group. Taken together, in this study we investigated the effects of EGCG in the regulation of mitochondrial integrity and antioxidative enzyme activity of HDF, suggesting that EGCG can be considered one of the possible antiaging reagents in the future. © 2007 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.freeradbiomed.2007.11.023
dc.sourceScopus
dc.subjectAging
dc.subjectAntioxidative enzyme
dc.subjectEGCG
dc.subjectHDF
dc.subjectMitochondrial potential
dc.subjectROS
dc.typeArticle
dc.contributor.departmentOTOLARYNGOLOGY
dc.description.doi10.1016/j.freeradbiomed.2007.11.023
dc.description.sourcetitleFree Radical Biology and Medicine
dc.description.volume44
dc.description.issue6
dc.description.page1032-1041
dc.identifier.isiut000254275300012
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