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|Title:||Sensitive and rapid method to quantify icaritin and desmethylicaritin in human serum using gas chromatography-mass spectrometry||Authors:||Shen, P.
Gas chromatography-mass spectrometry (GC-MS)
Selective ion monitoring (SIM) mode
|Issue Date:||2007||Citation:||Shen, P., Wong, S.P., Yong, E.L. (2007). Sensitive and rapid method to quantify icaritin and desmethylicaritin in human serum using gas chromatography-mass spectrometry. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 857 (1) : 47-52. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2007.06.026||Abstract:||The prenyl-flavones, icaritin and desmethylicaritin, are bioactive compounds from the traditional Chinese medicinal herb, Epimedium, extracts of which can enhance bone health in animal models. In order to examine their bioavailability in humans, we have developed and validated a sensitive method to quantify icaritin and desmethylicaritin in human sera, using gas chromatography-mass spectrometry. The serum samples were extracted with ethyl acetate and then derivatized with BSTFA in pyridine (4:1). With genistein as internal standard, calibration curves with good linearity (R2 > 0.99) within the concentration range of 0.15-10 nM in the selective ion monitoring mode were obtained. The limits of detection and quantization were 11 and 33 pM for icaritin, and 23 and 70 pM for desmethylicaritin, respectively; inter- and intra-assay variabilities were <15%, and accuracies were between 89 and 110%. Icaritin, but not desmethylicaritin, was detected from 1 h, increasing to a peak at 8 h (1.51 ± 1.6 nM) in sera of human volunteers after ingestion of an aqueous decoction of Epimedium. This sensitive method can be used to quantify serum levels of icaritin and desmethylicaritin for pharmacokinetic studies. © 2007 Elsevier B.V. All rights reserved.||Source Title:||Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences||URI:||http://scholarbank.nus.edu.sg/handle/10635/26004||ISSN:||15700232||DOI:||10.1016/j.jchromb.2007.06.026|
|Appears in Collections:||Staff Publications|
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