Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.fertnstert.2008.10.030
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dc.titleComparative evaluation of different in vitro systems that stimulate germ cell differentiation in human embryonic stem cells
dc.contributor.authorRichards, M.
dc.contributor.authorFong, C.-Y.
dc.contributor.authorBongso, A.
dc.date.accessioned2011-09-15T08:21:33Z
dc.date.available2011-09-15T08:21:33Z
dc.date.issued2010
dc.identifier.citationRichards, M., Fong, C.-Y., Bongso, A. (2010). Comparative evaluation of different in vitro systems that stimulate germ cell differentiation in human embryonic stem cells. Fertility and Sterility 93 (3) : 986-994. ScholarBank@NUS Repository. https://doi.org/10.1016/j.fertnstert.2008.10.030
dc.identifier.issn00150282
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/25981
dc.description.abstractObjective: To explore several culture systems that may prove efficient in driving human embryonic stem cells (hESCs) toward a germ cell lineage. Design: Embryoid bodies (EBs) derived from a female hESC line [HES-3 (XX)] and male hESC line [HES-4 (XY)] were cultured in six different culture conditions: [1] mitotically inactivated porcine ovarian fibroblasts (POF), [2] 100% conditioned medium from POF, [3] 50% conditioned medium from POF, [4] forskolin, [5] trans-retinoic acid (RA), and [6] forskolin and RA. Setting: Department of Obstetrics and Gynecology, National University of Singapore Research Laboratories, Singapore. Patient(s): None. Intervention(s): None. Main Outcome Measure(s): None. Result(s): Expression data for both HES-3 and HES-4 differentiating cultures strongly indicated that inactivated POFs encouraged differentiation of hESC EBs into a germ cell lineage. VASA and other germ cell markers were found to be elevated in all six culture conditions. Conclusion(s): Overall, POFs proved to be the best system for initiating germ cell differentiation, as shown by increases in the expression of several germ cell marker genes in EBs that were cocultured with POFs. © 2010 American Society for Reproductive Medicine.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.fertnstert.2008.10.030
dc.sourceScopus
dc.subjectCoculture
dc.subjectdifferentiation
dc.subjectgerm cell
dc.subjecthuman embryonic stem cell
dc.subjectVasa
dc.typeArticle
dc.contributor.departmentOBSTETRICS & GYNAECOLOGY
dc.description.doi10.1016/j.fertnstert.2008.10.030
dc.description.sourcetitleFertility and Sterility
dc.description.volume93
dc.description.issue3
dc.description.page986-994
dc.identifier.isiut000274957300044
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