Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bone.2009.03.674
DC FieldValue
dc.titleThe fabrication of nano-hydroxyapatite on PLGA and PLGA/collagen nanofibrous composite scaffolds and their effects in osteoblastic behavior for bone tissue engineering
dc.contributor.authorNgiam, M.
dc.contributor.authorLiao, S.
dc.contributor.authorCheng, Z.
dc.contributor.authorChan, C.K.
dc.contributor.authorRamakrishna, S.
dc.contributor.authorPatil, A.J.
dc.date.accessioned2011-08-03T01:51:21Z
dc.date.available2011-08-03T01:51:21Z
dc.date.issued2009
dc.identifier.citationNgiam, M., Liao, S., Cheng, Z., Chan, C.K., Ramakrishna, S., Patil, A.J. (2009). The fabrication of nano-hydroxyapatite on PLGA and PLGA/collagen nanofibrous composite scaffolds and their effects in osteoblastic behavior for bone tissue engineering. Bone 45 (1) : 4-16. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bone.2009.03.674
dc.identifier.issn87563282
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/25347
dc.description.abstractBone is a nanocomposite consisting of two main components, nano-hydroxyapatite (n-HA) and Type I collagen (Col). The aim is to exploit the nano-scale functional and material characteristics of natural bone in order to modulate cellular functions for optimal bone repair in bone graft systems. Here, we present an effective and novel technique in obtaining n-HA in cognate with native apatite on electrospun nanofibers within minutes without any pre-treatment. Using an alternate calcium and phosphate (Ca-P) solution dipping method, n-HA was formed on poly(lactide-co-glycolide) acid (PLGA) and blended PLGA/Col nanofibers. The presence of the functional groups of collagen significantly hastened n-HA deposition closed to nine-fold. The quantity of n-HA impinged upon the specific surface area, whereby mineralized PLGA/Col had a greater surface area than non-mineralized PLGA/Col, whereas n-HA did not significantly improve the specific surface area of mineralized PLGA compared to pure PLGA. The novelty of the process was that n-HA on PLGA had a positive modulation on early osteoblast capture (within minutes) compared to pure PLGA. Contrary, cell capture on mineralized PLGA/Col was comparable to pure PLGA/Col. Interestingly, although n-HA impeded proliferation during the culture period (days 1, 4 and 7), the cell functionality such as alkaline phosphatase (ALP) and protein expressions were ameliorated on mineralized nanofibers. The amount of n-HA appeared to have a greater effect on the early stages of osteoblast behavior (cell attachment and proliferation) rather than the immediate/late stages (proliferation and differentiation). © 2009 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bone.2009.03.674
dc.sourceScopus
dc.subjectBone graft
dc.subjectBone tissue engineering
dc.subjectHydroxyapatite
dc.subjectNanocomposite
dc.subjectOsteoblast
dc.typeArticle
dc.contributor.departmentMECHANICAL ENGINEERING
dc.contributor.departmentORTHOPAEDIC SURGERY
dc.contributor.departmentDIVISION OF BIOENGINEERING
dc.description.doi10.1016/j.bone.2009.03.674
dc.description.sourcetitleBone
dc.description.volume45
dc.description.issue1
dc.description.page4-16
dc.identifier.isiut000267036700001
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