Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2010.01.004
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dc.titleA bFGF-releasing silk/PLGA-based biohybrid scaffold for ligament/tendon tissue engineering using mesenchymal progenitor cells
dc.contributor.authorSahoo, S.
dc.contributor.authorGoh, J.C.H.
dc.contributor.authorToh, S.L.
dc.date.accessioned2011-08-03T01:51:19Z
dc.date.available2011-08-03T01:51:19Z
dc.date.issued2010
dc.identifier.citationSahoo, S., Goh, J.C.H., Toh, S.L. (2010). A bFGF-releasing silk/PLGA-based biohybrid scaffold for ligament/tendon tissue engineering using mesenchymal progenitor cells. Biomaterials 31 (11) : 2990-2998. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2010.01.004
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/25345
dc.description.abstractAn ideal scaffold that provides a combination of suitable mechanical properties along with biological signals is required for successful ligament/tendon regeneration in mesenchymal stem cell-based tissue engineering strategies. Among the various fibre-based scaffolds that have been used, hybrid fibrous scaffolds comprising both microfibres and nanofibres have been recently shown to be particularly promising. This study developed a biohybrid fibrous scaffold system by coating bioactive bFGF-releasing ultrafine PLGA fibres over mechanically robust slowly-degrading degummed knitted microfibrous silk scaffolds. On the ECM-like biomimetic architecture of ultrafine fibres, sustained release of bFGF mimicked the ECM in function, initially stimulating mesenchymal progenitor cell (MPC) proliferation, and subsequently, their tenogeneic differentiation. The biohybrid scaffold system not only facilitated MPC attachment and promoted cell proliferation, with cells growing both on ultrafine PLGA fibres and silk microfibres, but also stimulated tenogeneic differentiation of seeded MPCs. Upregulated gene expression of ligament/tendon-specific ECM proteins and increased collagen production likely contributed to enhancing mechanical properties of the constructs, generating a ligament/tendon analogue that has the potential to be used to repair injured ligaments/tendons. © 2010 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2010.01.004
dc.sourceScopus
dc.subjectbFGF
dc.subjectBiomimetic scaffolds
dc.subjectElectrospinning
dc.subjectLigament & tendon
dc.subjectMesenchymal progenitor cells
dc.subjectSilk
dc.typeArticle
dc.contributor.departmentMECHANICAL ENGINEERING
dc.contributor.departmentORTHOPAEDIC SURGERY
dc.description.doi10.1016/j.biomaterials.2010.01.004
dc.description.sourcetitleBiomaterials
dc.description.volume31
dc.description.issue11
dc.description.page2990-2998
dc.identifier.isiut000275946500005
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