Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2008.04.012
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dc.titleIn vivo study of anterior cruciate ligament regeneration using mesenchymal stem cells and silk scaffold
dc.contributor.authorFan, H.
dc.contributor.authorLiu, H.
dc.contributor.authorWong, E.J.W.
dc.contributor.authorGoh, J.C.H.
dc.contributor.authorToh, S.L.
dc.date.accessioned2011-08-03T01:47:40Z
dc.date.available2011-08-03T01:47:40Z
dc.date.issued2008
dc.identifier.citationFan, H., Liu, H., Wong, E.J.W., Goh, J.C.H., Toh, S.L. (2008). In vivo study of anterior cruciate ligament regeneration using mesenchymal stem cells and silk scaffold. Biomaterials 29 (23) : 3324-3337. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2008.04.012
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/25287
dc.description.abstractAlthough most in vitro studies indicate that silk is a suitable biomaterial for ligament tissue engineering, in vivo studies of implanted silk scaffolds for ligament reconstruction are still lacking. The objective of this study is to investigate anterior cruciate ligament (ACL) regeneration using mesenchymal stem cells (MSCs) and silk scaffold. The scaffold was fabricated by incorporating microporous silk sponges into knitted silk mesh, which mimicked the structures of ligament extracellular matrix (ECM). In vitro culture demonstrated that MSCs on scaffolds proliferated vigorously and produced abundant collagen. The transcription levels of ligament-specific genes also increased with time. Then MSCs/scaffold was implanted to regenerate ACL in vivo. After 24 weeks, histology observation showed that MSCs were distributed throughout the regenerated ligament and exhibited fibroblast morphology. The key ligament ECM components including collagen I, collagen III, and tenascin-C were produced prominently. Furthermore, direct ligament-bone insertion with typical four zones (bone, mineralized fibrocartilage, fibrocartilage, ligament) was reconstructed, which resembled the native structure of ACL-bone insertion. The tensile strength of regenerated ligament also met the mechanical requirements. Moreover, its histological grading score was significantly higher than that of control. In conclusion, the results imply that silk scaffold has great potentials in future clinical applications. © 2008 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2008.04.012
dc.sourceScopus
dc.subjectLigament
dc.subjectMesenchymal stem cells
dc.subjectRegeneration
dc.subjectSilk
dc.subjectTissue engineering
dc.typeArticle
dc.contributor.departmentORTHOPAEDIC SURGERY
dc.description.doi10.1016/j.biomaterials.2008.04.012
dc.description.sourcetitleBiomaterials
dc.description.volume29
dc.description.issue23
dc.description.page3324-3337
dc.identifier.isiut000257344600005
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