Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biocel.2009.12.024
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dc.titleThe fourth isoform of the adenine nucleotide translocator inhibits mitochondrial apoptosis in cancer cells
dc.contributor.authorGallerne, C.
dc.contributor.authorTouat, Z.
dc.contributor.authorMartel, C.
dc.contributor.authorMayola, E.
dc.contributor.authorSharaf, el dein O.
dc.contributor.authorLe, Bras M.
dc.contributor.authorLemaire, C.
dc.contributor.authorBrenner, C.
dc.contributor.authorChen, Z.X.
dc.contributor.authorPervaiz, S.
dc.contributor.authorBuron, N.
dc.contributor.authorJacotot, E.
dc.contributor.authorBorgne-Sanchez, A.
dc.contributor.authorLemoine, A.
dc.date.accessioned2011-07-27T06:39:05Z
dc.date.available2011-07-27T06:39:05Z
dc.date.issued2010
dc.identifier.citationGallerne, C., Touat, Z., Martel, C., Mayola, E., Sharaf, el dein O., Le, Bras M., Lemaire, C., Brenner, C., Chen, Z.X., Pervaiz, S., Buron, N., Jacotot, E., Borgne-Sanchez, A., Lemoine, A. (2010). The fourth isoform of the adenine nucleotide translocator inhibits mitochondrial apoptosis in cancer cells. International Journal of Biochemistry and Cell Biology 42 (5) : 623-629. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biocel.2009.12.024
dc.identifier.issn13572725
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/24966
dc.description.abstractThe adenine nucleotide translocator (ANT) is a mitochondrial bi-functional protein, which catalyzes the exchange of ADP and ATP between cytosol and mitochondria and participates in many models of mitochondrial apoptosis. The human adenine nucleotide translocator sub-family is composed of four isoforms, namely ANT1-4, encoded by four nuclear genes, whose expression is highly regulated. Previous studies have revealed that ANT1 and 3 induce mitochondrial apoptosis, whereas ANT2 is anti-apoptotic. However, the role of the recently identified isoform ANT4 in the apoptotic pathway has not yet been elucidated. Here, we investigated the effects of stable heterologous expression of the ANT4 on proliferation, mitochondrial respiration and cell death in human cancer cells, using ANT3 as a control of pro-apoptotic isoform. As expected, ANT3 enhanced mitochondria-mediated apoptosis in response to lonidamine, a mitochondriotoxic chemotherapeutic drug, and staurosporine, a protein kinase inhibitor. Our results also indicate that the pro-apoptotic effect of ANT3 was accompanied by decreased rate of cell proliferation, alteration in the mitochondrial network topology, and decreased reactive oxygen species production. Of note, we demonstrate for the first time that ANT4 enhanced cell growth without impacting mitochondrial network or respiration. Moreover, ANT4 differentially regulated the intracellular levels of hydrogen peroxide without affecting superoxide anion levels. Finally, stable ANT4 overexpression protected cancer cells from lonidamine and staurosporine apoptosis in a manner independent of Bcl-2 expression. These data highlight a hitherto undefined cytoprotective activity of ANT4, and provide a novel dichotomy in the human ANT isoform sub-family with ANT1 and 3 isoforms functioning as pro-apoptotic while ANT2 and 4 isoforms render cells resistant to death inducing stimuli. © 2010 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biocel.2009.12.024
dc.sourceScopus
dc.subjectADP/ATP carrier
dc.subjectBcl-2
dc.subjectCell death
dc.subjectMitochondria
dc.typeArticle
dc.contributor.departmentPHYSIOLOGY
dc.description.doi10.1016/j.biocel.2009.12.024
dc.description.sourcetitleInternational Journal of Biochemistry and Cell Biology
dc.description.volume42
dc.description.issue5
dc.description.page623-629
dc.identifier.isiut000276324400011
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