Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.urology.2009.11.024
DC Field | Value | |
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dc.title | Expression of hepaCAM and Its Effect on Proliferation of Tumor Cells in Renal Cell Carcinoma | |
dc.contributor.author | Xun, C. | |
dc.contributor.author | Luo, C. | |
dc.contributor.author | Zhang, Q. | |
dc.contributor.author | Yan, L. | |
dc.contributor.author | Wu, X. | |
dc.contributor.author | Shen, S. | |
dc.date.accessioned | 2011-07-27T06:38:16Z | |
dc.date.available | 2011-07-27T06:38:16Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Xun, C., Luo, C., Zhang, Q., Yan, L., Wu, X., Shen, S. (2010). Expression of hepaCAM and Its Effect on Proliferation of Tumor Cells in Renal Cell Carcinoma. Urology 75 (4) : 828-834. ScholarBank@NUS Repository. https://doi.org/10.1016/j.urology.2009.11.024 | |
dc.identifier.issn | 00904295 | |
dc.identifier.issn | 15279995 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/24915 | |
dc.description.abstract | Objectives: To evaluate hepaCAM (hepatocyte cell adhesion molecule) gene expression in patients with renal cell carcinoma (RCC) and to explore its effect on proliferation of 786-0 cells. hepaCAM is a tumor suppressor gene, which has been identified as a member of immunoglobulin superfamily cell adhesion molecule. Methods: Two-step reverse transcription-polymerase chain reaction was used to determine hepaCAM expression in 30 paired (RCC and the adjacent non-RCC) renal specimens. Transfection studies were carried out by expressing green fluorescent protein and green fluorescent protein-fused hepaCAM in 786-0 cells. Results: Significant downregulation of hepaCAM was detected in 25 of 30 RCC patients tested. When transfected into 786-0 cells, the number of colony formation was reduced by 5-fold according to colony formation assay. MTT (3-diphenyltetrazolium bromide) showed the inhibition rates on the fourth, fifth, and sixth days of culturing were 26.5%, 38.1%, and 35.7%, respectively. Conclusion: Our data show that hepaCAM is frequently downregulated in RCC, and that exogenous hepaCAM exhibits antiproliferative effect on 786-0 cells, suggesting that silencing of hepaCAM may be associated with carcinogenesis of RCC. © 2010 Elsevier Inc. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.urology.2009.11.024 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | PHYSIOLOGY | |
dc.description.doi | 10.1016/j.urology.2009.11.024 | |
dc.description.sourcetitle | Urology | |
dc.description.volume | 75 | |
dc.description.issue | 4 | |
dc.description.page | 828-834 | |
dc.identifier.isiut | 000276258300012 | |
Appears in Collections: | Staff Publications |
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