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Title: Interaction of the immunoglobulin-like cell adhesion molecule hepaCAM with caveolin-1
Authors: Moh, M.C. 
Lee, L.H.
Zhang, T.
Shen, S. 
Keywords: Caveolae
Immunoglobulin-like cell adhesion molecule
Lipid rafts
Issue Date: 2009
Citation: Moh, M.C., Lee, L.H., Zhang, T., Shen, S. (2009). Interaction of the immunoglobulin-like cell adhesion molecule hepaCAM with caveolin-1. Biochemical and Biophysical Research Communications 378 (4) : 755-760. ScholarBank@NUS Repository.
Abstract: Subsequent to our identification of the novel immunoglobulin-like cell adhesion molecule hepaCAM, we demonstrated that hepaCAM is capable of modulating cell growth and cell-extracellular matrix interactions. In this study, we examined the localization of hepaCAM in lipid rafts/caveolae as well as the interaction of hepaCAM with the caveolar structural protein caveolin-1 (Cav-1). Our results revealed that a portion of hepaCAM resided in detergent-resistant membranes and co-partitioned with Cav-1 to low buoyant density fractions characteristic of lipid rafts/caveolae. In addition, co-localization and coimmunoprecipitation assays confirmed the association of hepaCAM with Cav-1. Deletion analysis of hepaCAM showed that the extracellular first immunoglobulin domain of hepaCAM was required for binding Cav-1. Furthermore, when co-expressed, Cav-1 induced the expression of hepaCAM as well as distributed hepaCAM to intracellular Cav-1-positive caveolar structures. Taken together, our findings indicate that hepaCAM is partially localized in the lipid rafts/caveolae and interacts with Cav-1 through its first immunoglobulin domain. © 2008 Elsevier Inc. All rights reserved.
Source Title: Biochemical and Biophysical Research Communications
ISSN: 0006291X
DOI: 10.1016/j.bbrc.2008.11.119
Appears in Collections:Staff Publications

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